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Updated: May 28, 2026

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

Detergent-Based Decellularization Preserves Extracellular Matrix Ultrastructure in Ovine Soft Tissues.

Ibrahim E Helal¹, Mahmoud F Ahmed^2,3, Ahmed M Abdellatif⁴

¹Department of Agriculture, Faculty of Environment Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia.

Biomimetics (Basel, Switzerland)

|May 26, 2026

Summary

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This study developed decellularized extracellular matrix (dECM) scaffolds from ovine tissues using a detergent protocol. The resulting dECM scaffolds preserve ECM structure and show potential for tissue engineering applications.

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Xenogeneic decellularized extracellular matrix (dECM) scaffolds are promising for tissue engineering.
Ovine tissues offer a potential source for dECM scaffold development.

Purpose of the Study:

To develop and characterize dECM scaffolds from ovine skin, tunica vaginalis, fascia lata, and pericardium.
To evaluate the efficiency of a detergent-based decellularization protocol for ECM preservation.

Main Methods:

Sequential detergent-based decellularization using sodium dodecyl sulfate and Triton X-100.
Evaluation through gross examination, histology, SEM, and residual DNA quantification.

Main Results:

Keywords:

biomaterials decellularization extracellular matrix ovine scanning electron microscopy tissue engineering

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Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

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Last Updated: May 28, 2026

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Decellularized tissues showed increased translucency and reduced pigmentation, indicating effective cell removal.

Histology and SEM confirmed preservation of collagen-rich ECM structure and removal of cellular components.

Approximately 94% reduction in residual DNA content was achieved across all tissues.

Conclusions:

The detergent-based protocol reliably produces structurally preserved, acellular scaffolds from multiple ovine tissues.
These dECM scaffolds possess structural characteristics suitable for potential tissue engineering applications.