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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: May 28, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Imaging genome dynamics in real time with CRISPR-based technologies.

Benjamin G Adams1, Jiahui Wu2

  • 1Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA, 01003, USA.

Current Opinion in Chemical Biology
|May 26, 2026
PubMed
Summary

CRISPR-Cas technology revolutionizes live-cell genome imaging, enabling visualization of dynamic interactions crucial for gene regulation and understanding cellular function and disease.

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Related Experiment Videos

Last Updated: May 28, 2026

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Gene expression is central to cellular functions, biological processes, and diseases.
  • Dynamic interactions between genomic loci are critical for gene regulation.
  • Visualizing these spatiotemporal dynamics is essential for understanding their function.

Purpose of the Study:

  • To review recent advancements in CRISPR-based genome imaging techniques.
  • To highlight methods for background reduction, signal amplification, and guide RNA tiling.
  • To discuss complementary fluorescence microscopy techniques.

Main Methods:

  • CRISPR-Cas technology for dynamic genome imaging.
  • Development of methods for live-cell imaging of single and multiple loci.
  • Techniques for background reduction and signal amplification.
  • Guide RNA tiling strategies.
  • Integration with fluorescence microscopy.

Main Results:

  • CRISPR-based methods offer powerful tools for dynamic genome imaging.
  • Recent advancements improve resolution, sensitivity, and multiplexing capabilities.
  • These techniques allow real-time observation of genomic locus interactions.

Conclusions:

  • CRISPR-based genome imaging is a rapidly advancing field with significant potential.
  • These technologies are vital for elucidating the role of genome organization in cellular function and disease.
  • Future developments will likely enhance the spatiotemporal resolution and applicability of these imaging methods.