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Updated: May 28, 2026

Isolation of Primary Murine Brain Microvascular Endothelial Cells
08:14

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Published on: November 14, 2014

The Endocytic Receptor DEC205 Is Expressed by Brain Endothelial Cells and Is Involved in Regulating the Blood-Brain

Sonali Singh1, Sabine Ring1, Xuan Lei1

  • 1Department of Dermatology, University Hospital Heidelberg, Im Neuenheimer Feld 440, 69120 Heidelberg, Germany.

Cells
|May 27, 2026
PubMed

The surface receptor DEC205 (alias CD205, NLDC-145, Ly75) has long been a marker for murine dendritic cells and Langerhans cells and functions as receptor to facilitate antigen uptake. We revisited the tissue expression pattern of DEC205 in mice and revealed expression by endothelial cells (ECs) in the central nervous system. Using the murine brain-derived EC line bEnd.3, we could show that DEC205 serves as an endocytic receptor that directs potential ligands to lysosomal compartments. As for its function in ECs, upon engagement by anti-DEC205 antibodies, DEC205 seems to guide Claudin 5, a component of endothelial junctions, away from the surface of ECs to a LAMP-1+, degradative compartment. This opens endothelial junctions, eventually allowing leukocytes to migrate from blood to tissue sites. In aggregate, by regulating the blood-brain barrier, DEC205 in ECs may contribute to the regulation of inflammatory processes in the central nervous system.

Keywords:
DEC205blood–brain barrierendothelial cells

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