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Updated: May 28, 2026

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

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Published on: February 22, 2020

Natural Killer T Cell Function in Lymphoma Patients.

Roshanak Derakhshandeh^1,2, Michael S Lee^1,2, Yuyi Zhu^1,2

¹Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21202, USA.

|May 27, 2026

Summary

This summary is machine-generated.

Natural killer T (NKT) cells are crucial for anti-tumor immunity. This study found impaired NKT cell function and altered cytokine profiles in lymphoma patients, with potential biomarkers identified for prognosis.

Keywords:

NKT cells T-cells cancer immunotherapy cytokines lymphocytes lymphoma

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Published on: February 8, 2022

Area of Science:

Immunology
Oncology
Cell Biology

Background:

Natural killer T (NKT) cells are key players in bridging innate and adaptive immunity, essential for anti-tumor responses.
Dysregulation of NKT cells and cytokine profiles is implicated in various cancers, including lymphoma.

Purpose of the Study:

To evaluate NKT and T cell function in lymphoma patients.
To identify correlations between specific cytokines, immune cell function, and clinical outcomes in lymphoma.

Main Methods:

Histological confirmation of diagnoses.
Flow cytometry and artificial antigen-presenting cells (aAPCs) for NKT/T cell function assessment.
ELISA and quantitative RT-PCR (qPCR) for cytokine analysis, including plasma and mRNA levels.

Main Results:

NKT cell activation was significantly reduced in lymphoma patients (44%) compared to healthy donors (82%).
Lymphoma patients exhibited elevated circulating pro-inflammatory (IL-6) and anti-inflammatory (IL-10, Sema4D) cytokines.
NKT cell function in peripheral blood correlated with bone marrow NKT cell function, indicating systemic immune status.

Conclusions:

Impaired NKT cell function and altered cytokine profiles are characteristic of lymphoma.
Sema4D may serve as a prognostic biomarker for B cell lymphoma, associated with disease progression and immune dysfunction.
aAPC-qPCR offers a rapid method for assessing immune cell function in cancer patients.