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Updated: May 28, 2026
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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
Published on: March 29, 2012
Machine Learning-Based Identification of Hub Genes and Temporal Regulation Mechanisms in Zebrafish Fin Regeneration
Xiaoying Jiang1, Junli Zheng1, Yuqin Shu1
1State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Genes
|May 27, 2026
Summary
This study identifies key genes regulating zebrafish fin regeneration and their connection to immune cells. These findings offer new targets for understanding tissue repair in vertebrates.
Area of Science:
- Regenerative Biology
- Immunology
- Genomics
Background:
- Zebrafish fin regeneration is a model for vertebrate tissue repair.
- Regulatory networks and immune microenvironment interactions are not fully understood.
Purpose of the Study:
- Identify hub genes in zebrafish fin regeneration.
- Elucidate molecular mechanisms and immune dynamics.
- Investigate crosstalk between regulatory networks and immunity.
Main Methods:
- Integrated bulk RNA-seq datasets.
- Applied differential expression analysis, WGCNA, and machine learning.
- Performed functional enrichment, TO-GCN, immune infiltration analysis, and RT-qPCR.
Main Results:
- Identified 82 candidate genes and 10 hub genes related to cytoskeleton, ECM, and focal adhesion.
- Uncovered hierarchical gene regulation and functional switching.
- Found correlations between hub genes and immune cell infiltration (B cells, macrophages, CD8+ T cells).
Conclusions:
- Provides potential gene targets for zebrafish fin regeneration.
- Offers insights into regulatory networks and immune microenvironment crosstalk.
- Valuable reference for vertebrate tissue regeneration studies.
