Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 28, 2026

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

Engineering a Compartmentalized Multi-Cell Co-Culture Hydrogel System Using Beeswax/Fucoidan/Alginate for Cultured

Jihad Kamel¹, Jun-Yeong Lee¹, Sadia Afrin¹

¹College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea.

Foods (Basel, Switzerland)

|May 27, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stent-Assisted Surgical Creation of a Ductal Drainage Tract in the Third Eyelid Gland for Treatment of Postoperative Lacrimal Cyst of the Nictitating Membrane in a Dog.

Veterinary ophthalmology·2026

Same author

Two Cases of Fibrin Membrane-Induced Pupillary Block Treated With Neodymium-Doped Yttrium Aluminium Garnet (Nd:YAG) Laser After Cataract Surgery in Dogs.

Veterinary medicine and science·2026

Same author

Hyaluronic Acid-Based Photocurable Glue Application as an Alternative Surgical Approach for Corneal Injuries in Canine Patients: A Case Series.

Veterinary medicine and science·2026

Same author

Recent advancements in cellulose-based hydrogel crosslinking using high-energy ionizing radiation for biomedical applications: a focused review.

Journal of biomaterials science. Polymer edition·2026

Same author

LXW7 Peptide Modification of Acellular Liver Scaffolds Improves Endothelialization and Hemocompatibility in Bioengineered Liver.

Journal of functional biomaterials·2026

Same author

A heparin-functionalized scaffold loaded with pleiotrophin enhances endothelialization and angiogenic potential in liver tissue engineering.

Biomaterials science·2026

Same journal

The Potential for Bioactive Peptide Production in a Fermented Dairy Beverage Based on Chickpea Water Extract Using Proteolytic Lactic Acid Bacteria.

Foods (Basel, Switzerland)·2026

Same journal

Influence of Protein Concentration on Heat-Induced Fouling of Oat Drink.

Foods (Basel, Switzerland)·2026

Same journal

Microalgae as Future Foods: Unlocking Their Potential and Overcoming Barriers to Market Adoption and Commercialization.

Foods (Basel, Switzerland)·2026

Same journal

Effect of High-Intensity Ultrasound and Calcium Chelation on Functional Properties of Casein Micelles.

Foods (Basel, Switzerland)·2026

Same journal

GC-MS and GC-IMS Based Metabolomics Combined with Cellular Assays to Characterize Volatile Compounds and Pharmacological Activity of <i>Lysimachia foenum-graecum</i> Hance from Different Origins.

Foods (Basel, Switzerland)·2026

Same journal

Research on the Potential Mechanism of Guanine Nucleotides Enhancing the Tolerance of <i>Lactiplantibacillus plantarum</i> Y12.

Foods (Basel, Switzerland)·2026

See all related articles

This summary is machine-generated.

Researchers developed a novel tri-culture hydrogel disc using beeswax/alginate and fucoidan/alginate. This biomaterial platform supports spatial organization of multiple cell types for advanced cultured meat research.

Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Background:

Developing effective biomaterial platforms is crucial for advancing cultured meat research.
Existing 3D co-culture systems often lack the spatial organization needed for complex cell interactions.

Purpose of the Study:

To establish a compartmentalized tri-culture hydrogel disc as a proof-of-concept in vitro model.
To incorporate a lipid-containing barrier phase for spatial cell organization.

Main Methods:

Fabrication and evaluation of beeswax/alginate (Bw/Algi) hydrogels for cytocompatibility.
Preparation and screening of fucoidan/alginate (Fu/Algi) hydrogels for C2C12 cell viability and differentiation.
Assembly of a composite Bw/Fu/Algi disc with compartmentalized myoblasts, fibroblasts, and endothelial cells, separated by Bw/Algi barriers.

Keywords:

C2C12 myoblasts alginate hydrogel beeswax fibroblast fucoidan indirect co-culture

More Related Videos

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Published on: July 30, 2020

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Published on: January 17, 2017

Related Experiment Videos

Last Updated: May 28, 2026

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Published on: August 7, 2016

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation

Published on: July 30, 2020

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Published on: January 17, 2017

Main Results:

Bw/Algi hydrogels supported cell attachment and growth.
Optimized fucoidan concentration in Fu/Algi hydrogels supported myogenic marker expression.
The Bw/Fu/Algi disc maintained viable, compartmentalized tri-culture, supporting indirect co-culture and demonstrating myogenic marker expression and protein production.

Conclusions:

A novel Bw/Fu/Algi tri-culture disc was successfully established, integrating a lipid barrier with hydrogel compartments.
This system provides a preliminary platform for multicellular in vitro modeling in cultured meat research.
The study offers insights into scaffold design for complex tissue development.