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Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
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Evolution of Rice Storage Quality and Underlying Microstructural Mechanisms Under Varying Nitrogen Fertilization

Fei Wen1, Jiahui Qi1, Haimiao Yang1

  • 1College of Advanced Agricultural Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.

Foods (Basel, Switzerland)
|May 27, 2026
PubMed
Summary

High nitrogen fertilizer application and long storage duration accelerate rice aging and off-flavor compound accumulation. Gel consistency (GC) is a key indicator of rice eating quality and storage tolerance.

Keywords:
indica-japonica hybrid ricenitrogen fertilizationquality stabilitystorage duration

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Area of Science:

  • Agricultural Science
  • Food Science
  • Materials Science

Background:

  • Nitrogen fertilizer rate and storage duration critically impact rice quality stability.
  • Understanding these factors is crucial for maintaining rice's milling, eating, and nutritional value.

Purpose of the Study:

  • To investigate the effects of nitrogen fertilizer levels and storage duration on rice quality.
  • To analyze changes in physicochemical properties, microstructure, and volatile profiles.
  • To identify key indicators for rice deterioration and storage tolerance.

Main Methods:

  • Three indica-japonica hybrid rice cultivars were used.
  • Four nitrogen fertilizer application levels were applied.
  • Rice quality was assessed after long-term storage, including microstructure analysis (SEM) and volatile profiling.

Main Results:

  • High nitrogen application (300 kg hm⁻²) induced structural defects in starch granules, accelerating aging.
  • Storage duration significantly altered volatile profiles and lipid degradation, amplified by high nitrogen.
  • Gel consistency (GC) correlated strongly with eating quality parameters and rice deterioration.

Conclusions:

  • High nitrogen fertilizer and extended storage negatively affect rice quality, promoting off-flavors.
  • Gel consistency (GC) can serve as an indirect indicator for rice deterioration and storage tolerance.
  • Cultivar-specific microstructural changes were observed under high nitrogen after storage.