Multi-Omics Analysis and In Vitro Experimental Validation Identify Candidate Mechanisms of Baicalein Against Chronic...

Chronic obstructive pulmonary disease (COPD) is characterized by persistent airflow limitation, chronic airway inflammation, and immune dysregulation, and currently available therapies remain insufficient to effectively halt disease progression. In this study, we used an integrative, hypothesis-generating strategy to investigate the potential mechanisms of baicalein against COPD by combining multi-dataset transcriptomic analysis, single-cell transcriptomics, machine learning-based feature selection, Mendelian randomization (MR), molecular simulation, virtual knockout analysis, and in vitro validation. Putative targets of baicalein were predicted using CTD, SEA, and SwissTargetPrediction, and were intersected with COPD-related genes collected from GeneCards and OMIM. Four GEO datasets (GSE20257, GSE42057, GSE76925, and GSE130928) were integrated after batch-effect correction, yielding a combined cohort of 260 control samples and 250 COPD samples. Candidate genes were prioritized by intersecting the results of LASSO regression, random forest, and support vector machine. Immune-cell infiltration was estimated using CIBERSORT, and single-cell transcriptomic data were used to define the cellular localization of prioritized genes. Formal protein-level MR analysis was conducted for CD163 using deCODE plasma protein pQTL/GWAS summary statistics as the exposure dataset and the IEU OpenGWAS COPD dataset (ebi-a-GCST90018807) as the outcome dataset. Molecular docking, molecular dynamics simulation, and virtual knockout analysis were further used to provide structural and network-level supportive evidence. Finally, LPS-stimulated BEAS-2B cells were used as an epithelial inflammatory model to evaluate the effects of baicalein by CCK-8 assay, wound-healing assay, ELISA, and RT-qPCR. Five core genes were prioritized, namely ABCC1, CD163, CYP1B1, IKBKB, and PIK3CA. Immune infiltration and single-cell analyses suggested that macrophage-associated immune regulation may represent an important mechanistic direction. MR analysis provided supportive genetic evidence for prioritizing CD163 in COPD. Molecular simulation offered preliminary structural support for several target-compound interactions. In LPS-stimulated BEAS-2B cells, baicalein reduced inflammatory cytokine release and modulated the expression of IKBKB, PIK3CA, IL1B, IL6, and IL10, thereby providing epithelial-level support for the predicted network. Taken together, these findings suggest that baicalein may exert anti-inflammatory effects in COPD through a multi-target, immune-associated mechanism, with macrophage-related regulation and CD163 emerging as noteworthy candidate directions for further investigation. This study provides an integrative framework for target prioritization and mechanistic exploration, while the predicted macrophage-centered mechanisms still require dedicated validation in immune-cell and in vivo models.