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Related Experiment Video

Updated: May 28, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

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Published on: April 22, 2016

Cofactor Engineering Strategy of Food-Grade Microorganisms: Redox Homeostasis Regulation and Functional Components

Di Zhang1, Haoran Wang1, Xin Song1

  • 1Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

Microorganisms
|May 27, 2026
PubMed
Summary
This summary is machine-generated.

This review explores food-grade microbial cofactor engineering, focusing on nicotinamide adenine dinucleotide (NAD) and NADP. Optimizing these cofactors maintains cellular redox balance and boosts functional ingredient biosynthesis in metabolic engineering.

Keywords:
cofactor engineering strategyfood-grade microorganismsfunctional ingredientsmetabolic engineeringredox homeostasis

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Area of Science:

  • Microbial biotechnology
  • Metabolic engineering
  • Biochemical pathways

Background:

  • Food-grade microorganisms rely on nicotinamide adenine dinucleotide (NAD) and its phosphate form (NADP) for essential metabolic processes.
  • Disrupting the NAD(P)+/NAD(P)H balance in metabolic engineering impairs microbial growth and product synthesis.

Purpose of the Study:

  • To systematically review recent advancements in food-grade microbial cofactor engineering.
  • To analyze key strategies for maintaining redox homeostasis and enhancing functional ingredient biosynthesis.

Main Methods:

  • Examination of metabolic flux optimization strategies.
  • Analysis of cofactor regeneration systems in microbial hosts.
  • Review of synergistic pathways for cofactor management.

Main Results:

  • Perturbation of the NAD(P)+/NAD(P)H network critically affects cellular redox homeostasis.
  • Effective cofactor engineering strategies are crucial for microbial strain performance.
  • Optimized cofactor networks enhance the synthesis of valuable functional ingredients.

Conclusions:

  • Future research should integrate multi-omics and intelligent control for industrial biomanufacturing.
  • Innovative approaches are needed to overcome challenges in large-scale cofactor engineering.
  • This field provides theoretical support for advancing food biomanufacturing.