Ultrasensitive, Selectivity Detection of Mercury Ion Using a Novel Localized Surface Plasmon Resonance Biosensor

Mercury ion, a highly toxic and bioaccumulative heavy metal pollutant, poses significant risks to human health and ecosystems even at trace concentrations. Therefore, the development of highly sensitive and selective analytical methods for mercury ions is critically important to safeguard environmental integrity and human health. In this work, 4-mercaptopyridine-functionalized gold nanoparticles (4-MPY-AuNPs) were synthesized and subsequently immobilized onto quartz slides to fabricate a localized surface plasmon resonance (LSPR) sensor. Exploiting the selective coordination interaction between the pyridyl nitrogen atoms of 4-MPY and Hg²⁺, this LSPR sensor enables highly specific detection of Hg²⁺. Moreover, injecting a trace amount of 4-mercaptopyridine-functionalized AuNPs into the flow cell triggers the in situ formation of a surface-confined AuNP-Hg²⁺-AuNP sandwich architecture, thereby enhancing the sensor's sensitivity. Under the optimized conditions, the proposed method exhibited a linear dynamic range of 1 × 10^-9-6 × 10^-7 mol L^-1, with a correlation coefficient (R²) of 0.9917 and a limit of detection (LOD) of 3.2 × 10^-10 mol L^-1; the LOD of this method is one order of magnitude lower than the LODs reported in contemporary Hg²⁺ detection methods. This method exhibits high selectivity, sensitivity, cost-effectiveness, and is label-free, thereby demonstrating significant potential for environmental applications.