Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Distribution Reliability and Automation01:25

Distribution Reliability and Automation

Distribution reliability in electrical power systems is critical for ensuring an uninterrupted power supply to consumers at minimal cost. According to IEEE Standard Terms, reliability is the probability that a device will function without failure over a specified time period or amount of usage. For electric power distribution, this translates to maintaining continuous power supply and addressing customer concerns over power outages. Several indices, as defined by IEEE Standard 1366-2012, are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility and pharmacokinetic evaluation of a needle-free injector for delivering high concentration antibody formulations.

Bioengineering & translational medicine·2026
Same author

In-depth Characterization of Photo-Stressed Antibody-Drug Conjugates by Mass Spectrometry.

The AAPS journal·2026
Same author

Identifying Key Factors Affecting mRNA-Lipid Nanoparticles Drug Product Formulation Stability.

Nanomaterials (Basel, Switzerland)·2026
Same author

Comprehensive forced degradation study revealing diverse chemical and physical degradation pathways of AAV8.

Gene therapy·2026
Same author

A Novel Non-Aqueous Emulsification Platform to Fabricate Polymeric Microspheres for Sustained Delivery of Biologics.

Pharmaceutical research·2025
Same author

Ischemic Stroke Management in India: Insights from the Indian Registry of Stroke Care Quality.

Cerebrovascular diseases (Basel, Switzerland)·2025
Same journal

Correction: Mohite et al. Bioactive Compound-Fortified Nanomedicine in the Modulation of Reactive Oxygen Species and Enhancement of the Wound Healing Process: A Review. <i>Pharmaceutics</i> 2025, <i>17</i>, 855.

Pharmaceutics·2026
Same journal

Metal Nanoparticle-Reinforced Hydrogels Applied in the Inhibition of Clinical Pathogens: Structural Features, Mechanisms, and Biomedical Prospects.

Pharmaceutics·2026
Same journal

Development and Evaluation of a Physiologically Based Pharmacokinetic Model for Cipepofol Across Diverse Clinical Populations.

Pharmaceutics·2026
Same journal

Artificial Intelligence in Nanopharmaceutical Development: From Predictive Design to Clinical Translation.

Pharmaceutics·2026
Same journal

Textilinin-1, a Snake Venom-Derived Kunitz-Type Protease Inhibitor, Accelerates Wound Healing Through Anti-Inflammatory, Antibacterial, and Pro-Regenerative Activities.

Pharmaceutics·2026
Same journal

Metformin and cRGDfc-Modified Nanoparticles Loaded with Curcumin for Age-Related Macular Degeneration: In Vitro Pharmacodynamics and Molecular Mechanisms.

Pharmaceutics·2026
See all related articles

Related Experiment Video

Updated: May 28, 2026

A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform
05:51

A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform

Published on: May 3, 2024

A High-Throughput Automation Platform for Accelerated AAV Stability Optimization.

Shuai Li1, Xiaoyan Wang1, Li Zhi1

  • 1Drug Product Development and Technology, Regeneron Pharmaceuticals Inc., Tarrytown, NY 10591, USA.

Pharmaceutics
|May 27, 2026
PubMed
Summary
This summary is machine-generated.

A new automated platform rapidly develops stable adeno-associated virus (AAV) formulations for gene therapy. This high-throughput system screens numerous conditions to quickly identify optimal, stable AAV formulations.

Keywords:
AAVautomationformulationhigh-throughput screening

More Related Videos

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

Related Experiment Videos

Last Updated: May 28, 2026

A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform
05:51

A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform

Published on: May 3, 2024

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

Area of Science:

  • Gene therapy
  • Biopharmaceutical formulation
  • Recombinant adeno-associated virus (AAV) vector development

Background:

  • Stable adeno-associated virus (AAV) formulations are crucial for advancing gene therapy applications.
  • Diverse AAV serotypes and engineered capsids present formulation development challenges.
  • Traditional manual methods are insufficient for rapid characterization of large formulation design spaces.

Purpose of the Study:

  • To develop an innovative, high-throughput automation platform for efficient AAV formulation development.
  • To accelerate the discovery of stable AAV formulations.
  • To enable rapid characterization of extensive formulation design spaces.

Main Methods:

  • Implemented a high-throughput automation platform for rapid formulation preparation and AAV analytics.
  • The system prepares 96 distinct formulations in 40 minutes and completes AAV compounding in 20 minutes per plate.
  • Precise control over pH, buffer components, and AAV titers was maintained throughout the process.

Main Results:

  • Screened 128 formulations for AAV1 across various buffer systems, pH ranges, and excipient combinations.
  • Identified optimal stable high-titer AAV1 formulations (1.2 × 10^14 vg/mL).
  • Formulations demonstrated stability under frozen, refrigerated, and room temperature storage conditions.

Conclusions:

  • The automation platform significantly accelerates AAV formulation development.
  • This approach conserves valuable AAV material and enables systematic exploration of the formulation design space.
  • Robust and stable AAV formulations were identified in a timeframe significantly shorter than traditional methods.