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Related Experiment Video

Updated: May 28, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

Mucoadhesive Chitosan-Gellan Gum Nanoparticles for Rifampicin Delivery: Taguchi Optimization and In Vitro Release

Siu-Yin Cheung¹, Aldana Galiyeva¹, Yerkeblan Tazhbayev¹

¹Institute of Chemical Issues, Karaganda National Research University Academician Ye.A. Buketov, Karaganda 100028, Kazakhstan.

|May 27, 2026

Summary

This study developed mucoadhesive chitosan-gellan gum nanoparticles for enhanced rifampicin delivery, improving tuberculosis treatment. The novel synthesis using ethanol and Taguchi optimization yielded stable nanoparticles with prolonged drug release and increased mucoadhesion.

Keywords:

antituberculosis drug chitosan gellan gum nanoparticles polyelectrolyte complex coacervation polysaccharide rifampicin

Related Experiment Videos

Last Updated: May 28, 2026

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications

Published on: April 13, 2022

Area of Science:

Nanotechnology
Materials Science
Pharmaceutical Sciences

Background:

Tuberculosis treatment faces challenges with conventional rifampicin formulations due to stability issues and side effects.
Developing advanced drug delivery systems is crucial for improving therapeutic efficacy and patient compliance.

Purpose of the Study:

To synthesize and optimize mucoadhesive chitosan-gellan gum nanoparticles for enhanced rifampicin delivery.
To investigate the impact of ethanol incorporation and Taguchi optimization on nanoparticle characteristics and drug release.

Main Methods:

Rifampicin-loaded chitosan-gellan gum nanoparticles were prepared using polyelectrolyte complex coacervation.
The Taguchi method was employed to optimize key formulation parameters including polymer ratios, drug-to-polymer ratio, temperature, and ethanol volume.
Physicochemical properties, drug release kinetics, mucoadhesion, and antimycobacterial activity were evaluated.

Main Results:

Optimized nanoparticles exhibited desirable characteristics: narrow particle size distribution (PDI: 0.212 ± 0.021), average size (153 ± 3 nm), and good stability (zeta potential: 22.94 ± 1.30 mV).
Drug release was pH-dependent and prolonged, with nanoparticles showing enhanced mucoadhesion compared to pure rifampicin.
Spectroscopic and thermal analyses confirmed successful rifampicin incorporation into the polymer matrix, and the minimum inhibitory concentration against Mycobacterium tuberculosis was determined.

Conclusions:

Developed chitosan-gellan gum nanoparticles offer a promising mucoadhesive platform for rifampicin delivery.
The integration of ethanol and Taguchi optimization provides an effective strategy for controlling nanoparticle properties and enhancing drug delivery performance for tuberculosis treatment.