Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026

Same author

Nanomembrane-based microfluidic platform with embedded electrical pressure transducer for on-chip nanoparticle quantification.

Lab on a chip·2026

Same author

A human synovial tendon-on-a-chip models key features of peritendinous adhesions and offers a new approach methodology for testing anti-fibrotic drugs.

bioRxiv : the preprint server for biology·2026

Same author

Bacterial extracellular vesicles indirectly destabilize a human stem cell-derived blood-brain barrier on-chip through pro-inflammatory stimulation of immune cells.

Lab on a chip·2026

Same author

Modular Integration of Impedance Sensing for Real-Time Assessment of Barrier Integrity.

bioRxiv : the preprint server for biology·2026

Same author

Digital Immunoassays for Sensitive Quantification of Blood Biomarkers Using Solid-State Nanopores.

ACS nano·2026

Search research articles

Related Experiment Video

Updated: May 28, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Catch-and-display immunoassay for digital biomarker detection.

Yuxuan Liu¹, Samuel N Walker², Michael E Klaczko³

¹Materials Science, University of Rochester, Rochester, NY 14627, USA.

Nanoscale Horizons

|May 27, 2026

Summary

We developed a cost-effective Catch-and-Display Immunoassay (CAD-IA) using nanomembranes for sensitive biomarker detection. This lab-friendly digital immunoassay offers a practical solution for common research settings, enabling precise quantification of low-abundance analytes.

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Published on: October 13, 2023

Related Experiment Videos

Last Updated: May 28, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Published on: October 13, 2023

Area of Science:

Biotechnology
Nanotechnology
Analytical Chemistry

Background:

Digital immunoassays offer high sensitivity for detecting low-abundance biomarkers.
Widespread adoption is hindered by high costs and complex workflows of existing platforms.
There is a need for accessible, laboratory-friendly digital immunoassay solutions.

Purpose of the Study:

To introduce a cost-effective and laboratory-friendly digital immunoassay, the Catch-and-Display Immunoassay (CAD-IA).
To demonstrate CAD-IA's utility for detecting biomarkers in common research settings.
To validate CAD-IA's performance using a traumatic brain injury biomarker.

Main Methods:

Development of a nanomembrane-based Catch-and-Display Immunoassay (CAD-IA) using ultrathin silicon nitride membranes.
Utilizing pipette-powered filtration to capture fluorescent nanoparticles within membrane pores.
Quantification of fluorescent immunocomplexes via confocal microscopy for digital signal generation.
Implementation on the microfluidic device featuring an ultrathin silicon membrane for diagnostics (µSiM-DX) platform.

Main Results:

CAD-IA demonstrated consistent digital outputs and linear quantification over two orders of magnitude.
The assay maintained linearity in serum matrices.
Achieved a limit of detection (LoD) of 0.02 µg mL⁻¹ for S100B with tyramide signal amplification (TSA), suitable for clinical diagnostics.

Conclusions:

CAD-IA represents a novel, cost-effective, and laboratory-deployable digital immunoassay.
The technology provides a practical alternative to expensive commercial platforms for biomarker detection.
This proof-of-concept demonstrates the potential of CAD-IA for widespread use in research laboratories.