A Stability-Indicating RP-HPLC Method for Simultaneous Determination of Triclabendazole and Levamisole in Oral...

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Triclabendazole (TBZ) and levamisole (LEV) are crucial antiparasitic agents.
• Accurate quantification of these drugs in oral suspensions is vital for veterinary medicine.
• Existing analytical methods may lack the required speed, precision, or stability-indicating capabilities.

Purpose of the Study:

• To develop and validate a rapid, precise, and stability-indicating high-performance liquid chromatographic (HPLC) method.
• To quantify triclabendazole (TBZ) and levamisole (LEV) in oral suspension formulations.
• To ensure the method can distinguish active ingredients from potential degradation products.

Main Methods:

• High-performance liquid chromatography (HPLC) was employed.
• A mobile phase of acetonitrile, methanol, and water (50:40:10, v/v/v) at pH 4.6 was used.
• Detection was performed at 245 nm, with a flow rate of 1.0 mL/min.

Main Results:

• The method demonstrated excellent linearity (R² = 0.9999) within specified concentration ranges.
• Retention times for TBZ and LEV were approximately 3.116 and 1.385 min, respectively.
• The method successfully separated TBZ and LEV from their degradation products, confirming its stability-indicating nature.

Conclusions:

• The developed HPLC method is fast, simple, precise, and stability-indicating.
• It is suitable for the routine analysis and quality control of triclabendazole and levamisole in oral suspensions.
• The method's robustness ensures reliable results under minor variations in analytical conditions.