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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
What is Glycolysis?00:56

What is Glycolysis?

Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

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Related Experiment Video

Updated: May 29, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Exploring Small-Molecule Modulators of Precursor miRNA Processing Associated With Glycolysis.

Miho Fujiki1, Daito Koga1, Asako Seike-Murata2

  • 1Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, Kasuga, Fukuoka, Japan.

Chemmedchem
|May 27, 2026
PubMed
Summary
This summary is machine-generated.

Researchers screened 2320 compounds to find small molecules that bind precursor microRNAs (pre-miRNAs). Several compounds were identified that interact with pre-miRNAs and inhibit their processing by Dicer.

Keywords:
fluorescent indicator displacement (FID) assayhigh‐throughput screeningmicroRNA precursorsmall molecule

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Last Updated: May 29, 2026

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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs crucial for gene expression regulation.
  • They influence numerous physiological processes, with human miRNAs potentially regulating 60% of protein-coding genes.
  • Targeting miRNA biogenesis with small molecules offers a route to modulate cellular functions.

Purpose of the Study:

  • To identify small-molecule binders targeting precursor microRNAs (pre-miRNAs).
  • To evaluate the impact of identified compounds on Dicer-mediated pre-miRNA processing.

Main Methods:

  • High-throughput screening of 2320 known compounds using a fluorescent indicator displacement (FID) assay.
  • Two sequential FID screenings to identify candidate compounds interacting with pre-miRNAs.
  • Surface plasmon resonance (SPR) analysis to confirm compound binding to pre-miRNAs.
  • Assessment of compound effects on Dicer enzyme activity in pre-miRNA processing.

Main Results:

  • 34 candidate compounds were identified through sequential FID screenings.
  • SPR analysis confirmed the binding of candidate compounds to target pre-miRNAs.
  • Several compounds demonstrated inhibitory effects on Dicer-mediated pre-miRNA processing.

Conclusions:

  • This study successfully identified small molecules capable of binding to pre-miRNAs.
  • Several identified compounds inhibit the Dicer-mediated processing of pre-miRNAs.
  • These findings provide potential therapeutic leads for modulating miRNA biogenesis and function.