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Updated: May 29, 2026

Analysis and Specification of Starch Granule Size Distributions
Published on: March 4, 2021
From microstructural features to macroscopic properties: A multi-scale investigation on starch characteristics of
Kai Zhang1, Yan Gao2, Xiyue Ran1
1College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China; Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Southwest University, Beibei, Chongqing 400715, China; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops in Chongqing, Beibei, Chongqing 400715, China.
Abstract:
Comprehensive characterization of starch diversity in global potato germplasm is critical for targeted breeding and end-use applications. This study evaluated 137 potato germplasms from 16 countries across three environments, and significant genotypic and environmental variation was observed in tuber starch (9.358%-24.143%), amylose (14.882%-30.814%), amylopectin, sucrose, and reducing sugar contents (0.025%-0.888%). Multi-scale analyses were performed on 15 representative accessions. Scanning electron microscopy revealed considerable differences in starch granule shape, density, and size (5.465-51.000 μm) among fresh tubers. Extracted starches exhibited unimodal particle size distributions. FTIR-derived R₁₀₄₇/₁₀₂₂ values (1.127-1.214) indicated diverse short-range molecular order. All starch gels exhibited predominant elastic behavior (G' > G"). Granule shape correlated with internal structural order, whereas particle size distribution of extracted starch showed no significant correlation with fresh-tuber granule characteristics. These findings enhance understanding of potato starch diversity and provide a foundation for germplasm utilization and breeding for tailored starch properties.
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