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Related Experiment Video

Updated: May 29, 2026

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

A Twin-Fluid Atomization Method for Fabricating Ovalbumin-Loaded Gelatin Microcapsules.

Toru Taharabaru1, Ryohei Noge2, Ayumi Goto1

  • 1Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan.

Chemical & Pharmaceutical Bulletin
|May 27, 2026
PubMed
Summary

This study presents a novel crosslinker-free method for creating gelatin microcapsules using spray-droplet atomization. This technique preserves the biological activity of encapsulated antigens, offering a safer alternative for drug delivery applications.

Keywords:
antigengelatinmicrocapsuleprotein

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Last Updated: May 29, 2026

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Published on: April 21, 2021

Area of Science:

  • Biomaterials Engineering
  • Drug Delivery Systems
  • Protein Therapeutics

Background:

  • Gelatin is a preferred material for microcapsule fabrication due to its solvent-free processing capabilities.
  • Traditional gelatin microcapsules often rely on chemical crosslinkers, posing safety risks and potentially impacting the bioactivity of encapsulated substances.
  • There is a need for safer, crosslinker-free methods to maintain the integrity and function of sensitive biomolecules.

Purpose of the Study:

  • To develop and validate a crosslinker-free method for fabricating gelatin microcapsules.
  • To assess the structural integrity and bioactivity of encapsulated model antigens using this novel method.
  • To provide a safer alternative for microencapsulation of protein therapeutics and antigens.

Main Methods:

  • Utilized twin-fluid spray-droplet atomization with a full-cone nozzle for microcapsule fabrication.
  • Employed gelatin as the wall material without chemical crosslinking agents.
  • Loaded ovalbumin (OVA), a model protein antigen, into the microcapsules.

Main Results:

  • Successfully fabricated stable gelatin microcapsules without the use of chemical crosslinkers.
  • Encapsulated ovalbumin (OVA) demonstrated preserved in vivo antigenicity.
  • The biological function of the model antigen was maintained post-encapsulation.

Conclusions:

  • The developed crosslinker-free method using spray-droplet atomization is effective for gelatin microcapsule fabrication.
  • This approach successfully preserves the bioactivity of encapsulated antigens, crucial for therapeutic applications.
  • This technique offers a promising, safer alternative for the development of protein-based therapeutics and vaccines.