Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 29, 2026

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
09:57

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Engineering Design of Artificial Phase-Separating Proteins.

Yongjun Mao1, Lu Bao1, Gaoshuai Li1

  • 1College of Pharmaceutical Science, Zhejiang Key Laboratory of Green Manufacturing Technology for Chemical Drugs, Key Laboratory for Green Pharmaceutical Technologies and Equipment of Ministry of Education, Zhejiang University of Technology, Hangzhou, Zhejiang, P. R. China.

Biotechnology Journal
|May 28, 2026
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HighRes_Builder: improved access and modeling of noncanonical residues for protein structure prediction.

Briefings in bioinformatics·2026
Same author

Establishment of multiple predictive models for early identification of the dengue cases in outpatient healthcare systems.

Scientific reports·2026
Same author

Temperature-relied defense: CsMYBs-CsHT9 modules activate N-feruloylputrescine biosynthesis against anthracnose in tea (Camellia sinensis).

The Plant journal : for cell and molecular biology·2025
Same author

Microwave absorption of multi-component Co<sub><i>x</i></sub>Ni<sub><i>y</i></sub>O<sub><i>z</i></sub> decorated 3D-honeycomb ordered mesoporous carbon nanospheres enhanced by impedance matching.

Physical chemistry chemical physics : PCCP·2025
Same author

A nomogram to predict long COVID risk based on pre- and post-infection factors: Results from a cross-sectional study in South China.

Public health·2024
Same author

The auxin-responsive CsSPL9-CsGH3.4 module finely regulates auxin levels to suppress the development of adventitious roots in tea (Camellia sinensis).

The Plant journal : for cell and molecular biology·2024
Same journal

Molecular Mechanisms of Cellulase Biosynthesis in Trichoderma reesei: Regulatory Networks, Secretion Pathways, and Environmental Modulation.

Biotechnology journal·2026
Same journal

The Impact of Collection Protocol on the Yield and Purity of Mesenchymal Stem Cell-Derived Extracellular Vesicles Isolated From Serum-Free Media.

Biotechnology journal·2026
Same journal

Biochemical and Functional Characterization of a Novel GH46 Chitosanase for Efficient Chitooligosaccharide Synthesis.

Biotechnology journal·2026
Same journal

LaeA Orchestrates Iron-Heme Supply and P450 Catalytic Efficiency for Enhanced Echinocandin B Biosynthesis in Aspergillus nidulans.

Biotechnology journal·2026
Same journal

Emerging Bioengineering Technologies in Female Reproduction: Preclinical Advances, Translational Challenges, and Future Outlook.

Biotechnology journal·2026
Same journal

Multi-Enzyme Cascade Reaction of Crude Enzyme Strategy for the Economical and Efficient Bioconversion of Rebaudioside A to Rebaudioside M.

Biotechnology journal·2026
See all related articles

Scientists engineered artificial phase-separating proteins by developing new design rules. This work provides a roadmap for creating novel biomolecular condensates for biotechnology and medicine.

Area of Science:

  • Biochemistry and Molecular Biology
  • Biotechnology
  • Synthetic Biology

Background:

  • Liquid-liquid phase separation (LLPS) is crucial for cellular processes.
  • Understanding LLPS principles like multivalent interactions and intrinsically disordered regions (IDRs) is key.
  • Translating LLPS biophysics into engineering guidelines is challenging.

Purpose of the Study:

  • To present a design-oriented synthesis for engineering artificial phase-separating proteins.
  • To characterize physicochemical patterns governing LLPS behavior.
  • To propose modular design heuristics for creating novel phase-separating proteins.

Main Methods:

  • Curated dataset analysis to identify LLPS-associated physicochemical patterns.
  • Evaluation of computational predictors for LLPS behavior.
Keywords:
bioinformatic analysisengineering designphase‐separating proteinsstructure–function continuum

More Related Videos

Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

Related Experiment Videos

Last Updated: May 29, 2026

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
09:57

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

  • Development of modular design heuristics including sequence composition, valency control, and topological strategies.
  • Integration into a design-build-test-learn (DBTL) framework.

    • Main Results:

    • Identified physicochemical patterns linked to LLPS.
    • Revealed limitations of current computational predictors, especially for hydrophobicity-rich or modular repeat proteins.
    • Proposed actionable design heuristics for engineering phase-separating proteins.
    • Demonstrated applicability across diverse applications like catalysis and hydrogel formation.

    Conclusions:

    • Phase separation can be treated as an engineerable property, not just emergent.
    • The proposed heuristics and DBTL framework offer a practical roadmap for rational design.
    • Enables construction of artificial phase-separating proteins for biotechnological and biomedical applications.