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Updated: May 29, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Correction: A real-time PCR assay for detection of low Pneumocystis jirovecii levels

Susana Ruiz-Ruiz1,2, Carolina A Ponce3, Nicole Pesantes1,2

  • 1Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)-Salud Pública, València, Spain.

Frontiers in Microbiology
|May 28, 2026
PubMed

Keywords:
low P. jirovecii loadsmajor surface glycoproteinsmitochondrial large subunit rRNAreal-time PCRsimultaneous detection

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...