Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

46° Convegno Nazionale di Studi di Medicina Trasfusionale Rimini, 13-15 maggio 2026.

Blood transfusion = Trasfusione del sangue·2026
Same author

Prehospital whole blood after SWiFT and TOWAR: not superior, not settled.

Blood transfusion = Trasfusione del sangue·2026
Same author

Comparison of hyperspectral imaging and FTIR spectroscopy for microplastic polymer identification: Proposal of a scalable protocol validated in a 12-month river survey.

Talanta·2026
Same author

Targeting CD38 to reduce anti-HLA antibody levels: A new and effective option to be integrated into desensitization protocols?

Transplant immunology·2025
Same author

Awareness of anti-hepatitis C virus immunological status among individuals born between 1969 and 1989 participating in anti-hepatitis C virus screening.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2025
Same author

Stabilization of bronchoalveolar lavage cells for postponed flow cytometric analysis.

Cytometry. Part B, Clinical cytometry·2025
Same journal

Thrombocytopenia in Pregnancy: A 5-Year Analysis of Characteristics and Practices From a Tertiary Academic Center.

International journal of laboratory hematology·2026
Same journal

AI In Leukemia Diagnostics: Complementing the Pathologist's Role.

International journal of laboratory hematology·2026
Same journal

Advanced Molecular Analysis in Hemophilia A in a Single Step: Next Generation Sequencing (NGS) and Copy Number Variation (CNV) Analysis.

International journal of laboratory hematology·2026
Same journal

Overwhelming Post-Splenectomy Sepsis From Capnocytophaga canimorsus.

International journal of laboratory hematology·2026
Same journal

Evaluation of the BT-50 Automatic Quality Control Unit on the XN9000 Hematology Analyzer.

International journal of laboratory hematology·2026
Same journal

Acquired Myeloperoxidase Deficiency in MDS/MPN-NOS Presenting With Aberrant Muddy Sand-Like Granulocytes.

International journal of laboratory hematology·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

Design and Implementation of an Automated Interpretation Algorithm for Lupus Anticoagulant Functional Testing.

Chiara Novelli1, Arianna Gatti1, Flora Ierna1

  • 1Immunohematology and Transfusion Center, ASST Ovest Milanese, Legnano, Italy.

International Journal of Laboratory Hematology
|May 28, 2026
PubMed
Summary
This summary is machine-generated.

An automated algorithm for lupus anticoagulant (LA) testing standardized interpretation and improved efficiency in antiphospholipid syndrome (APS) diagnosis. This middleware-based system reduced workload and enhanced diagnostic accuracy in laboratory settings.

Keywords:
algorithmantiphospholipid syndromeblood coagulation testlupus anticoagulantsoftware

More Related Videos

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
08:50

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Related Experiment Videos

Last Updated: May 29, 2026

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
09:43

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
08:50

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Area of Science:

  • Clinical diagnostics
  • Laboratory automation
  • Hematology

Background:

  • Lupus anticoagulant (LA) testing is crucial for diagnosing antiphospholipid syndrome (APS).
  • Current LA testing involves complex workflows with potential variability from anticoagulants and reagents.
  • Expert interpretation is often required due to these complexities.

Purpose of the Study:

  • To implement a middleware-based automated algorithm for LA testing.
  • To standardize interpretation and automate reflex testing for APS diagnosis.
  • To support clinical decision-making and reduce laboratory workload.

Main Methods:

  • Developed and implemented a rule-driven automated algorithm using the HemoHub system.
  • Incorporated good laboratory practice rules for reflex testing, interpretation, and auto-validation.
  • Validated the algorithm retrospectively on 190 cases and prospectively on 481 routine samples.

Main Results:

  • Achieved 100% concordance between automated and manual interpretations for LA negative and positive cases.
  • Automated interpretation provided useful comments when direct assignment was not possible.
  • 58.4% of samples were auto-validated, significantly reducing manual workload and interpretation time.

Conclusions:

  • The automated algorithm enhanced consistency and reduced interpretation time in LA testing.
  • Integration of clinical context and historical data improved diagnostic accuracy.
  • Middleware-based, rule-driven interpretation offers a reliable and efficient approach to standardize LA testing.