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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: May 31, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Single-cell and spatial RNA sequencing in prostate cancer.

Amin Ali1,2,3, Migle Mikutenaite4,5, Joachim Weischenfeldt4,5,6

  • 1Lancashire Teaching Hospitals NHS Foundation Trust, Preston, UK. amin.ali@lthtr.nhs.uk.

Nature Reviews. Urology
|May 28, 2026
PubMed
Summary

Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics reveal prostate cancer heterogeneity. These tools uncover cellular interactions and genomic changes driving disease, aiding biomarker and therapy development.

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Related Experiment Videos

Last Updated: May 31, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Area of Science:

  • Prostate biology and cancer research.

Background:

  • Single-cell RNA sequencing (scRNA-seq) is crucial for understanding prostate organ development, disease initiation, and progression.
  • scRNA-seq provides insights into benign prostatic hyperplasia and metastatic prostate cancer, focusing on the tumor microenvironment, cellular plasticity, and therapy resistance.

Purpose of the Study:

  • To explore the application of scRNA-seq and spatial transcriptomics in prostate cancer research.
  • To investigate cellular interactions, heterogeneity, and genomic alterations in prostate cancer.

Main Methods:

  • Utilizing single-cell RNA sequencing (scRNA-seq) to analyze cellular composition and interactions.
  • Employing spatial transcriptomics for high-resolution analysis of tumor architecture.
  • Integrating genomic and transcriptomic data for clonal evolution and therapy resistance analysis.

Main Results:

  • scRNA-seq identified complex interactions between epithelial, stromal, and immune cells driving disease heterogeneity.
  • Spatial transcriptomics enabled characterization of tumor architecture at single-cell resolution.
  • Bioinformatic inference of copy number variants facilitated integrated genomic-transcriptomic analysis.

Conclusions:

  • Understanding prostate cancer heterogeneity through these advanced techniques can support patient stratification.
  • Biomarker development and novel therapeutic strategies targeting genomic instability and tumor microenvironment interactions are potential outcomes.
  • These technologies offer a comprehensive view of prostate cancer biology, paving the way for personalized medicine.