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MiR-100-5p is involved in severe pneumonia in children by targeting MTOR

Lun Zhang1, Qing Wang2, Shoushan Mo3

  • 1Department of Pediatrics, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi City, China.

BMC Immunology
|May 28, 2026
PubMed

Insights

MicroRNA-100-5p (miR-100-5p) is a potential biomarker for severe childhood pneumonia. Its decreased expression is linked to the condition, suggesting a role in disease regulation.

Area of Science:

  • Pediatric Respiratory Medicine
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Severe pneumonia in children is a critical illness with significant mortality risks.
  • MicroRNA-100-5p (miR-100-5p) is investigated as a potential diagnostic marker for severe pediatric pneumonia.

Purpose of the Study:

  • To evaluate the diagnostic value of miR-100-5p in severe pneumonia among children.
  • To investigate the role of miR-100-5p in regulating cellular proliferation and inflammation in the context of pneumonia.

Main Methods:

  • Serum miR-100-5p expression was analyzed in 200 children.
  • In vitro studies involved WI-38 cells treated with lipopolysaccharide (LPS) to assess miR-100-5p function.
  • TargetScan and dual-luciferase reporter assays were used to identify and confirm MTOR as a downstream target of miR-100-5p.

Main Results:

  • miR-100-5p expression was significantly decreased in children with severe pneumonia, exhibiting high diagnostic accuracy.
  • miR-100-5p levels correlated with clinical indicators of disease severity.
  • Overexpression of miR-100-5p in LPS-stimulated cells enhanced proliferation and reduced inflammatory markers, while inhibition had opposite effects.
  • A negative association was observed between miR-100-5p and MTOR expression in pediatric severe pneumonia patients.

Conclusions:

  • miR-100-5p plays a role in severe childhood pneumonia by negatively regulating MTOR.
  • miR-100-5p demonstrates potential as a valuable biomarker for diagnosing severe pneumonia in children.
Abstract

Keywords:
BiomarkerChildrenMTORSevere pneumoniamiR-100-5p

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