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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: May 31, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
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Published on: August 26, 2009

Bivariate Cooperator-Catalyzed Hairpin Assembly for Amplifying Conformation-Guided Label-Free Ratiometric

Maolan He1, Jiayang He1, Min Long1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing Engineering Laboratory of Nanomaterials & Sensor Technologies, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

Analytical Chemistry
|May 29, 2026
PubMed
Summary
This summary is machine-generated.

A new bivariate cooperator-catalyzed hairpin assembly (bcCHA) strategy efficiently detects adenosine triphosphate (ATP) and miRNA-21 (miR-21) simultaneously. This label-free method improves accuracy and reduces background noise for sensitive biosensing.

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Area of Science:

  • Biomolecular Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Traditional hairpin assembly amplifiers face challenges with high background noise and low efficiency.
  • Simultaneous detection of multiple biomarkers requires highly specific and sensitive methods.

Purpose of the Study:

  • To develop a novel bivariate cooperator-catalyzed hairpin assembly (bcCHA) strategy for simultaneous and efficient detection of adenosine triphosphate (ATP) and miRNA-21 (miR-21).
  • To create a label-free, ratiometric fluorescence assay with improved selectivity and accuracy.

Main Methods:

  • Design of a functional birecognizable hairpin (brH) incorporating ATP aptamer and miR-21 complementary sequences.
  • Utilizing simultaneous hybridization to trigger hairpin disassembly and initiate bcCHA.
  • Employing repetitive strand migration and self-catalysis for signal amplification.
  • Incorporating dual-emissive silver nanoclusters as reporters for ratiometric fluorescence detection.

Main Results:

  • Achieved sensitive bivariate assay at the picomolar level with ratiometric fluorescence.
  • Demonstrated a bicooperator authentication mechanism for built-in error correction.
  • Successfully lowered false-positive background and enhanced selectivity and accuracy.

Conclusions:

  • The bcCHA strategy offers a simplified, label-free approach for multiplexed biomarker detection.
  • This method provides new insights for developing biomimetic platforms for biosensing, cell imaging, and diagnostics.