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Related Experiment Video

Updated: Jun 29, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Ratiometric Fluorescence Biosensor of Allosteric Ag Nanoclusters by G-Quadruplex-Aided Displacement Amplification.

Manlin Zhou1, Yuqing Zhang1, Zhihan Zhang1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing Engineering Laboratory of Nanomaterials & Sensor Technologies, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

ACS Sensors
|June 28, 2026
PubMed
Summary

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We developed a G-quadruplex folding-aided displacement amplification (G4DA) strategy for sensitive detection. This method uses bicolor fluorescent reporters for rapid and accurate analysis without enzymes.

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Conventional displacement reactions face challenges with high background noise and slow kinetics.
  • Existing methods often require enzymes or complex chemical modifications.

Purpose of the Study:

  • To introduce a novel G-quadruplex folding-aided displacement amplification (G4DA) strategy.
  • To enable rapid and sensitive detection of specific triggers using ratiometric fluorescence.

Main Methods:

  • Utilized allosteric silver nanoclusters (Ag NCs) as bicolor reporters (aAg554 and aAg610).
  • Employed a modular hairpin with a blocked recognition element, unlocked by a key effector.
  • Leveraged G-quadruplex (G4) structures for accelerated strand-exchange and signal amplification.
Keywords:
G-quadruplex foldingG4-aided displacement amplificationallosteric Ag nanoclustersratiometric fluorescence biosensortarget recycling

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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

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Last Updated: Jun 29, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Main Results:

  • Achieved ratiometric fluorescence signaling through a switch from red (aAg610) to green (aAg554) emission.
  • Demonstrated rapid reaction rates and high sensitivity due to G4-accelerated amplification.
  • Successfully detected a specific targeting trigger (tT) with minimal background.

Conclusions:

  • The G4DA strategy offers a simplified, enzyme-free approach for biosensing.
  • This method provides a new paradigm for biosensor development, bioanalysis, and potential therapeutic applications.
  • Highlights the potential of G4 structures in enhancing displacement reaction kinetics and sensitivity.