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Related Concept Videos

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

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Related Experiment Video

Updated: May 31, 2026

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Published on: October 1, 2008

NeuRoDev resolves lifelong temporal and cellular variation in human cortical gene expression.

Asia Zonca1, Erik Bot1, Jose Davila-Velderrain1

  • 1Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy.

Cell Reports
|May 29, 2026
PubMed
Summary

NeuRoDev is a new computational resource that analyzes over one million single-cell transcriptomes to map human brain development. This tool helps researchers understand neuronal and glial cell maturation dynamics.

Keywords:
CP: neurosciencebraincortexdevelopmentglianetworksneurogenesisneuronsingle-celltranscriptomics

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Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

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Related Experiment Videos

Last Updated: May 31, 2026

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Published on: October 1, 2008

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
10:12

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Published on: August 9, 2017

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Published on: May 8, 2020

Area of Science:

  • Neuroscience
  • Genomics
  • Computational Biology

Background:

  • Human brain development requires analyzing individual human cells.
  • Single-cell atlases offer insights into cellular states during brain development.
  • Technical limitations hinder the full potential of current single-cell atlases.

Purpose of the Study:

  • To present NeuRoDev, a computational resource for analyzing human brain development.
  • To provide curated transcriptomic data and analytical tools for neuronal and glial development research.
  • To establish a framework for interrogating cellular maturation and gene function.

Main Methods:

  • Compression of approximately 1 million single-cell transcriptomes into integrative summary networks.
  • Development of reproducible cell clustering to capture temporal and cellular variation.
  • Application of NeuRoDev to analyze developmental variation, cell genesis, and maturation dynamics.

Main Results:

  • NeuRoDev successfully integrates large-scale single-cell transcriptomic data.
  • Identified reproducible cell clusters representing distinct developmental stages and cell types.
  • Enabled detailed reconstruction of neuronal and glial cell genesis and maturation.

Conclusions:

  • NeuRoDev serves as a valuable reference framework for human brain development research.
  • The resource facilitates the interpretation of experimental organoid models.
  • NeuRoDev is freely available as software and web applications for interactive analysis.