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Updated: May 31, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Effect of five selected flavonoids on the stability of anthocyanins: Kinetics, structural characterization, and
Yini Fu1, Yafei Dong1, Jifang Miao1
1Zhejiang University Zhongyuan Institute, Zhengzhou 450001, Henan, PR China.
Abstract:
To address the issue of poor stability of anthocyanins, this study conducted a comparative investigation into the intermolecular copigmentation characteristics between five different flavonoids (catechin, luteolin, quercetin, eriodictyol, and taxifolin) and cyanidin-3-o-glucoside (C3G). The experimental results revealed that the redshift occurred in the maximum absorption wavelength upon the formation of the copigmentation. The copigmentation system with the addition of quercetin exhibited a high absorbance (1.14 ± 0.02) (p<0.05). The redness value also increased (a⁎ = 39.75). Further analysis demonstrated that the copigmentation reactions were all non-spontaneous endothermic reactions. The analysis of thermodynamic parameters indicated that structural differences among the flavonoids could influence the effectiveness of the copigmenting agents. Additionally, the incorporation of flavonoids enhanced the antioxidant capacity of C3G. Molecular interaction mechanism analysis indicates that hydrogen bonding and π-π stacking play key roles in the interaction between C3G and flavonoids, serving as the core driving forces of the copigmentation effect.
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