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Updated: Jun 1, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Fluorescent covalent organic framework and multivalent aptamer-driven magnetic nanoplatform for sensitive detection
Shihao Huang1,2, Hao Shen3,4, Li Ding5,6
1Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China.
Abstract:
Analytical platforms capable of rapid detection of foodborne pathogens with high sensitivity and localized specificity are indispensable not only for quality control protocols in the food industry but also broader applications in clinical diagnostics, public health surveillance, and environmental monitoring. Here, we engineered a novel fluorescently tagged magnetic nanoplatform for highly sensitive and specific detection of foodborne pathogens by leveraging an aptamer-functionalized covalent organic framework (COF) and integrating it with the rapid separation capabilities of magnetic nanoparticles to achieve significant signal amplification and target enrichment. Methicillin-resistant Staphylococcus aureus and Listeria monocytogenes were detected within 60 min after sample pretreatment with detection limits of 4 and 6 CFU/mL, respectively. Spiked recovery experiments in real food samples showed remarkable recovery (91.9%-106%), confirming potential for practical applications. Therefore, this work provides a novel and efficient COF-driven platform for the rapid detection of foodborne pathogens.
