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Updated: Jun 1, 2026

Green Synthesis, Characterization, Encapsulation, and Measurement of the Release Potential of Novel Alkali Lignin Micro-/Submicron Particles
Published on: March 1, 2024
Green-synthesized ε-polylysine/zein nanocomplexes as safe antimicrobials: Applications and metabolomic mechanisms in
Chunli Fang1, Ruiqi Zhu2, Jia Wei3
1School of Chemistry and Chemical Engineering/State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832000, Xinjiang, PR China; Institute of Food Science and Technology, Xinjiang Academy of Agricultural Science, Urumqi 830091, Xinjiang, PR China.
Abstract:
Microbial contamination is a major contributor to postharvest fruit losses, highlighting the need for effective and safe antimicrobial strategies. Herein, ε-polylysine (ε-PL)/zein nanocomplexes were fabricated via synergistic complexation using a flow-based flash nanoprecipitation (FNP) technique. The nanocomplexes showed negligible cytotoxicity and minimal surface residues after conventional washing. Additionally, the nanocomplexes exhibited uniform morphology, good storage stability over 14 days, and sustained release properties. More importantly, ε-PL/zein nanocomplexes demonstrated antimicrobial activity against both bacteria and fungi by disrupting cell integrity. As a proof-of-concept, grape preservation experiments showed that ε-PL/zein nanocomplexes significantly reduced decay rate (5.7%) compared with commercial ε-PL (10.5%) and the control (17.3%), while maintaining firmness and total soluble solids during storage (p < 0.05). Metabolomic analysis suggested nanocomplex treatment may be associated with changes in metabolic pathways related to stress resistance and quality maintenance. This work presents an innovative strategy for developing promising green antimicrobials for postharvest food preservation.
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