Related Experiment Video
Updated: Jun 2, 2026
06:40
In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
Published on: November 17, 2018
Dandelion-Derived Carbon Dots with pH-Responsive Charge and Dual ROS Regulation for Anti-Infection and Accelerated
Peili Li1,2,3,4, Siyu Zhang1, Haoran Xu1
1College of Chemistry and Materials Engineering, Anhui Science and Technology University, Bengbu 233000, China.
ACS Applied Materials & Interfaces
|June 1, 2026
Summary
Green synthesized dandelion carbon dots (DH-CDs) effectively treat bacterial wound infections by disrupting biofilms and promoting tissue repair. These biocompatible nanoparticles offer a promising solution for infected wounds with dual reactive oxygen species (ROS) regulation.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Wound Healing Research
Background:
- Bacterial infections and biofilms impede wound healing through antibiotic resistance and delayed tissue repair.
- Developing novel antimicrobial agents with wound healing capabilities is crucial for clinical applications.
Purpose of the Study:
- To fabricate dandelion herb-derived carbon dots (DH-CDs) as a biocompatible antimicrobial agent for wound healing.
- To investigate the physicochemical properties, antibacterial/antibiofilm activity, biocompatibility, and in vivo efficacy of DH-CDs.
Main Methods:
- Green solvothermal-extraction method for DH-CD synthesis.
- Physicochemical characterization (size, morphology, functional groups, pH-responsiveness).
- In vitro antibacterial/antibiofilm assays against S. aureus and E. coli.
- In vitro biocompatibility tests (hemolysis, fibroblast viability).
- In vivo wound healing study in a murine model.
Main Results:
- DH-CDs are quasi-spherical nanoparticles (4.87 nm) with pH-responsive properties.
- Broad-spectrum antibacterial activity (MICs 31.25-62.5 μg/mL) and mature biofilm disruption (300 μg/mL).
- Excellent biocompatibility (HC10 > 4000 μg/mL, >95% fibroblast viability).
- Dual ROS modulation: generates ROS to kill bacteria, scavenges ROS in mammalian cells.
- Accelerated wound closure in vivo, promoting re-epithelialization and tissue regeneration.
Conclusions:
- DH-CDs demonstrate potent antibacterial, antibiofilm, and wound healing properties.
- The dual ROS-modulating capability enhances efficacy and safety.
- DH-CDs represent a promising, green-synthesized nanomaterial for treating infected wounds.