Related Experiment Video
Updated: Jun 2, 2026

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
Study on the effects of different fermentation methods on fermented mustard microbial community composition and
Yaoying Zeng1, Yuheng Tan2, Le Xie3
1National Engineering Research Center for Rice and By-product Deep Processing, Hunan Key Laboratory of Processed Food for Special Medical Purpose, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
Abstract:
Fermented mustard is a traditional Chinese vegetable product with complex flavor profiles shaped primarily by microbial activity during fermentation. Although several studies focus on single fermentation systems, few have integrated microbial diversity and metabolomics. This study investigated the effects of three fermentation techniques, i.e., jar fermentation (JFM), pool fermentation (PFM), and bury fermentation (BFM), on the quality attributes, microbial community composition, and metabolite profile of fermented mustard. JFM was predominantly characterized by Lactobacillus and Wickerhamiella, creating a stable microaerobic environment that promoted lactic acid accumulation and ester synthesis. BFM showed higher abundance of potential pathogens such as Staphylococcus and Pseudomonas, suppressing lactic acid bacteria (LAB) activity. Metabolomics revealed 8447 metabolites, with lipids, organic heterocyclic compounds, and phenylpropanoids as key flavor contributors. These findings establish that JFM's microaerobic environment selectively enriches beneficial Lactobacillus-Wickerhamiella consortia while suppressing spoilage taxa, driving superior quality and safety through distinct metabolic pathways. This comparative framework provides targeted strategies for optimizing traditional mustard fermentation.
More Related Videos
Related Concept Videos
Microbes in the Production of Fermented Foods
Microbes in Food Production
Microbial Fermentation
Microbes in Beverage Production
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Methods to Assess Microbial Communities

