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Updated: Jun 3, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Wavefront imaging with low coherence illumination simplifies the optical diffraction tomography setup and improves
Abstract:
We present the employment of wavefront imaging using Quadriwave Lateral Shearing Interferometry (QLSI) for Optical Diffraction Tomography (ODT) reconstructions. Compared to a conventional Digital Holographic (DH) Interferometer, QLSI does not require a reference beam. It can also work efficiently with an incoherent source and hence reduces the speckle noise. Furthermore, QLSI is almost insensitive to phase wrapping, thus avoiding artefacts and easing the signal processing in ODT. We validate the method by reconstructing the 3D refractive index (3DRI) of a polystyrene bead via angle-scan diffraction tomography. The 3DRI reconstruction of the nano-printed phantom cell and the living yeast cell is also presented.
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