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Updated: Jun 3, 2026
Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
Preparation and antibacterial activities of silver nanoparticle-loaded hydroxyapatite/collagen bone-like
Masanori Kikuchi1, Taku Yoshida2,3,4, Yasushi Suetsugu2
1Bioceramics Group, Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, 1-1, Namiki, Tsukuba, 305- 0044, Ibaraki, Japan. KIKUCHI.Masanori@nims.go.jp.
Abstract:
A hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) loaded with silver nanoparticles (AgNPs) was prepared by immersion of HAp/Col powder in the AgNPs solution. The adsorption-desorption properties on the preparation process were investigated, followed by the evaluation of antibacterial activities and cytotoxicity. Adsorption of AgNPs on HAp/Col powder was almost finished at 240 min after mixing. Adsorption isotherm revealed that maximum adsorption amount was 1621.9 mg/g with the best fitted model, Dubinin-Radushkevich adsorption model. The AgNPs-loaded HAp/Col membrane prepared by immersion into 1.6 µg/mL and 6.3 µg/mL AgNPs colloidal solution demonstrated no survival of Escherichia coli and Staphylococcus aureus, respectively. Using human osteoblastic cell line, MG-63, no significant cytotoxicity was observed for the AgNPs-loaded HAp/Col membrane prepared with 6.3-12.5 µg/mL AgNPs colloidal solution. These results suggested that AgNPs-loaded HAp/Col would be a good candidate for antibacterial bone void fillers and coating materials for orthopedic and dental implants.
