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Related Experiment Video

Updated: Jun 3, 2026

Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria

Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria

Published on: July 6, 2013

An optimized engineered bacterium for tyrosinemia type 1 therapy: A multi-species preclinical study.

Peng Gu¹, Yunfei Zhao¹, Jiajia Hu¹

¹Department of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.

Molecular Therapy : the Journal of the American Society of Gene Therapy

|June 2, 2026

Summary

Engineered probiotics offer a novel treatment for hereditary tyrosinemia type 1 (HT1), a serious metabolic disorder. This innovative therapy effectively reduces toxic tyrosine buildup and improves disease symptoms in preclinical models.

Keywords:

engineered bacteria gut microbiota live bacterial therapeutics liver injury multi-species animal model synthetic biology tyrosinemia type 1

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Published on: September 26, 2020

Area of Science:

Biotechnology
Metabolic Disorders
Microbiome Therapeutics

Background:

Hereditary tyrosinemia type 1 (HT1) is a severe metabolic disorder characterized by toxic tyrosine accumulation.
Current treatments, nitisinone (NTBC) and diet, present lifelong burdens and side effects.

Purpose of the Study:

To develop and evaluate an engineered probiotic, e-EcN-HT, as a novel therapeutic for HT1.
To assess the efficacy and safety of e-EcN-HT across multiple animal models of HT1.

Main Methods:

Development of an optimized engineered probiotic (e-EcN-HT).
Preclinical testing in FAH-/- mice, FAH-/- rabbits, and Bama minipigs.
Evaluation of tyrosine metabolism and safety assessments.

Main Results:

e-EcN-HT mitigated acute and chronic manifestations of HT1 in mice and rabbits.
The probiotic demonstrated effective tyrosine metabolism in pigs.
Comprehensive safety assessments confirmed e-EcN-HT was well-tolerated without adverse effects.

Conclusions:

Engineered probiotics represent a promising therapeutic strategy for hereditary tyrosinemia type 1.
e-EcN-HT shows significant potential for treating HT1 and other metabolic disorders.