Related Experiment Video
Updated: Jun 3, 2026
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Associations Between Social Jetlag and Gut Microbiome Profiles in Adolescents
Qinghai Gong1, Keqin Ding1, Wangwei Lou2
1Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
Nature and Science of Sleep
|June 2, 2026
Summary
Sedentary lifestyle in adolescents is linked to specific gut bacteria shifts, not overall microbiome changes. Further research is needed to understand the health implications of this gut-brain axis connection.
Area of Science:
- Microbiome research
- Gut-brain axis
- Adolescent health
Background:
- The microbiota-gut-brain axis is crucial for health.
- Emerging evidence connects sedentary lifestyle (SJL) to the gut microbiome.
- This study examines SJL's impact on adolescent gut microbial composition.
Purpose of the Study:
- To investigate the association between self-reported SJL and gut microbiome diversity in adolescents.
- To explore correlations between SJL and specific bacterial taxa and functional genes.
- To determine if SJL influences the Firmicutes/Bacteroidetes ratio.
Main Methods:
- Recruited 120 healthy adolescents (mean age 12.69 years).
- Assessed self-reported SJL and analyzed gut microbial alpha-diversity, F/B ratio, taxa, and functional gene diversity.
- Compared microbiome composition between adolescents with SJL <2 h/d and ≥2 h/d.
Main Results:
- Continuous SJL negatively correlated with microbial alpha-diversity at the family level (P<0.05).
- Specific bacterial taxa and genera showed differential abundance related to SJL.
- The glutamate dehydrogenase gene (gdh_K00262) was enriched in the high SJL group after FDR correction (q=0.05).
Conclusions:
- Adolescent SJL is associated with shifts in functionally relevant gut taxa, not global microbiome restructuring.
- The specific mechanisms linking SJL and the gut microbiome require further investigation.
- Understanding this interaction is vital for adolescent health outcomes.
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