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Updated: Jun 3, 2026

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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Enhancing Triggered Release of Polar Cargo from ROS-Responsive Liposomes.

Annaleena Hansen1, Mayesha B Mustafa1, Jinchao Lou2

  • 1Department of Chemistry; University of Tennessee Knoxville, 1420 Circle Drive, Knoxville, Tennessee 37996, United States.

Bioconjugate Chemistry
|June 2, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed improved ROS-responsive liposomes for targeted drug delivery. These novel liposomes demonstrate faster, more efficient release of therapeutic cargo in response to reactive oxygen species (ROS), enhancing delivery to diseased cells.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Drug Delivery

Background:

  • Liposomes are versatile nanocarriers for therapeutic cargo delivery.
  • Improving triggered and targeted cargo release remains a key challenge in liposome technology.
  • Reactive oxygen species (ROS) levels differ significantly between diseased and healthy cells, offering a potential trigger mechanism.

Purpose of the Study:

  • To develop and characterize improved ROS-responsive liposomes with enhanced cargo release capabilities.
  • To engineer a novel lipid switch incorporating a boronate ester headgroup and a self-immolative linker (SIL).
  • To evaluate the efficiency and responsiveness of the new liposomes in releasing both hydrophobic and hydrophilic cargo.

Main Methods:

  • Synthesis of a novel ROS-responsive lipid switch (2) with a boronate ester headgroup and a methoxy-substituted SIL.
  • Formulation of liposomes incorporating the novel lipid switch.
  • Fluorescence-based dye release assays (hydrophobic and hydrophilic cargo).
  • Dynamic light scattering (DLS) for liposome size and dispersity analysis.

Main Results:

  • Liposomes formulated with lipid switch 2 demonstrated initial stability.
  • Successful triggered release of both hydrophobic and hydrophilic cargo upon exposure to hydrogen peroxide (H2O2).
  • Lipid 2 enabled significantly faster polar dye cargo release compared to previous designs, even at lower incorporation percentages.
  • DLS confirmed changes in liposome size and dispersity post-treatment.

Conclusions:

  • The improved ROS-responsive lipid switch (2) significantly enhances the speed and efficiency of liposome cargo release.
  • This advanced platform shows increased potential for ROS-targeted drug delivery applications.
  • The developed liposomes offer a promising strategy for selective delivery to diseased cells based on ROS levels.