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Updated: Jun 4, 2026
Orthotopic Small Bowel Transplantation in Rats
Published on: November 6, 2012
Protocol for heterotopic mouse intestinal transplantation to study transplant immunology
Hajime Matsushima1, Akihiko Soyama2, Koji Hashimoto3
1Department of Surgery, Division of Digestive Surgery and Transplantology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan; Department of General Surgery, Digestive Disease & Surgery Institute, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Abstract:
Heterotopic mouse intestinal transplantation is a valuable model for investigating intestinal tissue injury and host immune responses to intestinal allografts. Here, we present a detailed protocol for heterotopic mouse intestinal transplantation. We describe steps for donor surgery and preparation of an intestinal graft on the back table. We then detail procedures for heterotopic intestinal transplantation, including vascular anastomoses. For complete details on the use and execution of this protocol, please refer to Matsushima et al.1 and Fujita et al.2.
