Plasma-Activated Medium Suppresses Lung Cancer H1299 Cell Migration through the MAPK Pathway

This research aimed to investigate the effect of PAM on the migration of lung cancer cells. Lactate dehydrogenase assays, colony formation assays, invasion assays, cell cycle assays, and self-developed microfluidic chips were used to thoroughly investigate the effects of PAM on H1299 cells. The results indicated that PAM treatment significantly increased the toxicity to lung cancer cells and induced cell cycle arrest at the G2/M phase. Conversely, the MAPK signaling pathway was observed to be regulated in lung cancer cells; therefore, their proliferation and migration were inhibited. The results of the microfluidic chip analysis, as well as invasion and colony formation assays, further corroborated the inhibitory effect of PAM on the migration and proliferation of H1299 cancer cells. These results reveal that PAM influences the biological behavior of lung cancer cells through migration, thus providing a theoretical basis for its potential therapeutic effect on lung cancer.