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Updated: Jun 5, 2026

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Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development
Published on: February 28, 2014
BMP signaling during gastrulation pre-patterns the dorsal spinal cord
Biorxiv : the Preprint Server for Biology
|June 4, 2026
Summary
Bone morphogenetic protein (BMP) signaling patterns dorsal neural fates during gastrulation, not later in the spinal cord. This study revises the classic model of dorsal spinal cord development.
Area of Science:
- Neuroscience
- Developmental Biology
- Genetics
Background:
- The classic model suggests roofplate-derived Bone Morphogenetic Protein (BMP) patterns dorsal interneuron subtypes concentration-dependently.
- Genetic studies of BMP pathway components have yielded variable results, challenging the established model.
Purpose of the Study:
- To investigate the precise timing of BMP signaling in patterning dorsal neural fates in vivo.
- To re-evaluate the role of BMP signaling in spinal cord development.
Main Methods:
- Single-cell profiling
- Fate mapping
- Mosaic perturbations
Main Results:
- Dorsal neural fates are patterned by BMP signaling during gastrulation, contrary to the classic model.
- Post-neural tube BMP signaling has limited roles in domain specification and maturation.
- Dorsal progenitors originate from the ventral gastrula and adopt BMP-dependent transcriptional states.
Conclusions:
- Dorsal neural fates are initially patterned by gastrulation-stage BMP sources before roofplate induction.
- The classic model requires revision regarding the timing and primary source of BMP signaling in spinal cord patterning.
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