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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jun 6, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

STIMscope: centimeter-scale all-optical imaging and patterned optogenetic manipulation at single-cell resolution.

Hamid Chorsi1,2, Saray Soldado-Magraner3, Yan Jin1

  • 1Department of Neurology, University of California, Los Angeles, Los Angeles, CA, USA.

Biorxiv : the Preprint Server for Biology
|June 5, 2026
PubMed
Summary
This summary is machine-generated.

We developed the STIMscope, an affordable, open-source all-optical neuroscience platform. This system enables cellular-resolution imaging and optogenetic manipulation across large fields of view, making advanced neuroscience research more accessible.

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Last Updated: Jun 6, 2026

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Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Area of Science:

  • Neuroscience
  • Optical Engineering
  • Biotechnology

Background:

  • All-optical probing enables linking cellular observation to intervention.
  • Existing platforms are complex, expensive, and have limited fields of view.
  • This restricts their use in studying large neuronal populations.

Purpose of the Study:

  • To develop an accessible, cost-effective all-optical platform for cellular-resolution neuroscience.
  • To overcome the limitations of existing complex and expensive systems.
  • To enable large-field-of-view imaging and optogenetic manipulation.

Main Methods:

  • Developed the Spatiotemporal Illumination Microscope (STIMscope), a benchtop platform with tandem optics, a CMOS sensor, and a digital micromirror device.
  • Integrated a GPU-based processing unit and microcontroller for hardware synchronization.
  • Created the Closed-loop ready Real-time Imaging and Stimulation Pipeline (CRISPI) for accelerated processing and control.

Main Results:

  • STIMscope achieves cellular-scale resolution with large fields of view (up to 14 mm × 11 mm).
  • CRISPI offers low latency (91.6 ms imaging-to-stimulation loop).
  • Validated in fixed brain tissue, neuronal cultures, and organotypic slices, demonstrating stimulus decoding and short-term memory dynamics.

Conclusions:

  • STIMscope provides a cost-effective (<$5,000 USD) and open-source solution for all-optical neuroscience.
  • It democratizes advanced neuroscience research by reducing technical barriers.
  • The platform supports routine cellular-resolution experiments for a wider range of laboratories.