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Related Experiment Video

Updated: Jun 6, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

Fasciclin 2 Cooperates with Discs Large to Maintain Epithelial Architecture.

Tara M Finegan^1,2, Michael W Linhoff¹, Hannah Rice¹

¹Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA.

Biorxiv : the Preprint Server for Biology

|June 5, 2026

Summary

Cell adhesion molecules like Fasciclin 2 (Fas2) regulate tissue development. This study reveals Fas2 uses distinct modes to help cells reintegrate, with scaffold protein Discs large (Dlg1) stabilizing Fas2 for effective epithelial repair.

Related Experiment Videos

Last Updated: Jun 6, 2026

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

Area of Science:

Developmental Biology
Cell Biology
Molecular Biology

Background:

Cell adhesion molecules of the immunoglobulin superfamily (IgCAMs) are crucial for tissue morphogenesis.
Epithelial maintenance in *Drosophila* relies on cell reintegration, where displaced cells reincorporate into the monolayer.
Fasciclin 2 (Fas2) and Neuroglian (Nrg) were previously identified as parallel regulators of reintegration, but intracellular mechanisms were unknown.

Purpose of the Study:

To elucidate the intracellular mechanisms by which Fas2 regulates epithelial reintegration.
To identify functional intracellular partners of transmembrane Fas2.
To understand the interplay between Fas2 and Nrg in epithelial maintenance.

Main Methods:

Yeast two-hybrid screening to identify protein interactions.
Genetic analysis in *Drosophila* follicular epithelium.
Fluorescence recovery after photobleaching (FRAP) to assess protein dynamics.

Main Results:

Fas2 regulates reintegration through both transmembrane and GPI-linked modes, with the transmembrane mode being more effective.
The scaffold protein Discs large (Dlg1) was identified as an intracellular partner of transmembrane Fas2.
Dlg1 disruption increases Fas2 mobility, indicating it stabilizes a cortical pool of Fas2, primarily within the Fas2-dependent reintegration pathway.

Conclusions:

Epithelial reintegration depends on coordinated adhesion-scaffold coupling.
Dlg1 plays a key role in stabilizing transmembrane Fas2 at the cell cortex, facilitating epithelial repair.
Mechanistic parallels exist between epithelial reintegration and IgCAM-dependent processes in nervous system development.