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Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
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Bioprospecting microbial proteases: methods and multisector applications.

Aradhana Satapathy1, Gagan Kumar Panigrahi1

  • 1Department of Zoology, School of Applied Sciences, Centurion University of Technology and Management, Bhubaneswar, India.

Preparative Biochemistry & Biotechnology
|June 5, 2026
PubMed
Summary
This summary is machine-generated.

Microbial proteases are key hydrolase enzymes with diverse industrial uses. This review highlights their role in sustainable applications like waste valorization and bioremediation.

Keywords:
Advanced technologydetectionenzyme assayindustrial applicationisolationmicrobial proteasesprotein engineeringscreening

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Area of Science:

  • Biochemistry and Biotechnology
  • Enzymology
  • Industrial Microbiology

Background:

  • Proteases (proteinases/peptidases) are hydrolase enzymes cleaving peptide bonds.
  • They are classified as endo-peptidases or exo-peptidases.
  • Microbial alkaline proteases are vital for pharmaceuticals, detergents, food processing, and waste management.

Purpose of the Study:

  • To provide a comprehensive overview of microbial proteases.
  • To integrate advanced screening, optimization, and downstream processing.
  • To highlight their potential in the circular bio-economy and environmental sustainability.

Main Methods:

  • Review of recent advancements in strain improvement (adaptive evolution).
  • Analysis of environmentally friendly production strategies.
  • Exploration of protease utilization in waste valorization and by-product enhancement.

Main Results:

  • Consolidation of recent progress in microbial protease research.
  • Demonstration of interdisciplinary potential across environmental, agricultural, and industrial sectors.
  • Summary of classification, production, optimization, screening, and applications.

Conclusions:

  • Microbial proteases offer significant potential for sustainable industrial processing.
  • Their applications in bioremediation and waste management are expanding.
  • Future innovations in bio-catalysis are anticipated.