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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 6, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Machine Learning-Assisted Surface Ligand Engineering Strategy for Enhanced Sensitivity of Immunoassay Platform.

Jinbo Cao1, Tiemei Li2, Yao Wang2

  • 1Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, GDMPA Key Laboratory for Process Control and Quality Evaluation of Chiral Pharmaceuticals, School of Chemistry, South China Normal University, Guangzhou 510006, Guangdong, P. R. China.

Analytical Chemistry
|June 5, 2026
PubMed
Summary
This summary is machine-generated.

We developed a new nanozyme (D-PtPdOs) with enhanced catalytic activity for detecting Pseudomonas aeruginosa. This nanozyme, combined with machine learning, enables ultrasensitive and intelligent point-of-care diagnostics.

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jun 6, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Nanozymes offer potential for point-of-care (POC) diagnostics but suffer from limited catalytic activity.
  • Surface engineering is crucial for enhancing nanozyme performance in clinical applications.

Purpose of the Study:

  • To develop a trimetallic nanozyme (D-PtPdOs) with superior peroxidase-like activity via surface charge-transfer ligand modulation.
  • To integrate the enhanced nanozyme with machine learning (ML) algorithms for ultrasensitive and intelligent detection of *Pseudomonas aeruginosa* ( *P. aeruginosa*).

Main Methods:

  • Surface engineering of trimetallic nanoparticles using charge-transfer ligands (d-histidine) to tune electron density.
  • Density functional theory (DFT) calculations to elucidate the mechanism of enhanced catalytic activity.
  • Integration of the D-PtPdOs nanozyme into enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA) platforms.
  • Application of ML algorithms for high-precision classification and quantitative prediction of *P. aeruginosa* infection levels.

Main Results:

  • The D-PtPdOs nanozyme exhibited significantly enhanced peroxidase-like activity compared to natural horseradish peroxidase (HRP).
  • DFT calculations confirmed enhanced H2O2 adsorption and reduced activation energy barrier due to d-histidine modification.
  • D-PtPdOs-based ELISA and LFIA platforms achieved 14.58-fold and 250-fold sensitivity enhancements, respectively, over conventional methods.
  • ML integration enabled accurate *P. aeruginosa* detection in complex human blood samples, mitigating matrix interference.

Conclusions:

  • Surface ligand engineering is a viable strategy to overcome catalytic limitations in nanozymes.
  • The D-PtPdOs nanozyme offers a promising alternative to HRP for ultrasensitive immunoassays.
  • The integration of nanozymes, ML, and immunoassays advances the development of intelligent POC diagnostic platforms.