Protocol for a murine transient middle cerebral artery occlusion model with local intra-arterial drug delivery

Here, we present a protocol for a murine transient middle cerebral artery occlusion (tMCAO) model incorporating an intra-arterial catheter for local drug delivery during reperfusion. We describe steps for pre-operative preparation, surgical procedure, and post-operative care. We detail procedures for the insertion of a microcatheter for targeted drug delivery during reperfusion to mimic clinical mechanical thrombectomy. This approach enables investigation of adjunct therapies for ischemia-reperfusion injury. For complete details on the use and execution of this protocol, please refer to Lee et al.¹.