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PmPPO-mediated drought adaptation mechanism in Prunus mira Koehne: Interaction with PmRad23d and regulation of ROS
Jianxin Li1, Xin Bao2, Yong Zhao2
1College of Horticulture, College of Forestry and Grassland Science, Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, Jilin Agricultural University, Changchun, 130118, China.
Abstract:
Global warming has resulted in frequent droughts worldwide, significantly affecting plant normal growth and development. Polyphenol oxidase (PPO), a copper-containing redox enzyme in plants, serves multiple functions. However, limited research has investigated the role of PPO in regulating plant drought adaptation. In this study, PmPPO was isolated from Prunus mira Koehne, the wild ancestor of cultivated peaches, which is a rare tree species known for its high stress tolerance under extreme conditions. Our results indicated that PmPPO was preferentially expressed in young roots, with its expression significantly induced by drought and abscisic acid (ABA). Overexpression of PmPPO in plants demonstrated enhanced tolerance to drought stress. Under drought stress, transgenic plants exhibited increased antioxidant enzyme activity, improved reactive oxygen species (ROS) scavenging capacity, and reduced cellular damage compared to wild-type (WT). Moreover, PmPPO overexpression facilitated anthocyanin accumulation by regulating the expression of genes involved in anthocyanin biosynthesis following drought treatment. Additionally, yeast two-hybrid (Y2H) and Luciferase complementation imaging (LCI) assays confirmed interactions between PmPPO and both PmRad23d and PmRGLG2. These findings introduce new molecular targets for genetic manipulation in peach germplasm improvement and present potential candidate genes for screening stress tolerance through molecular breeding.
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