Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Leaching

Microbial Leaching

Microbial leaching, also known as bioleaching, is an environmentally favorable method for extracting metals from low-grade ores using specific microorganisms. This biotechnological approach is particularly valuable for mining operations targeting copper, gold, and uranium, where traditional extraction methods may be economically or environmentally impractical.Copper Leaching and Microbial CatalysisIn copper bioleaching, crushed ore is arranged into heaps and irrigated with a dilute sulfuric...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reversal of Pacing-Induced Cardiomyopathy After Upgrade to Left Bundle Branch Area Pacing: A Case Report.

Clinical case reports·2026

Same author

Probing the limits of genetic recoding using multi-omics-guided evolution.

Nature communications·2026

Same author

Global landscape of protein-coding and long non-coding RNA alternative splicing and regulation in the human esophageal squamous cell carcinoma.

Cancer cell international·2026

Same author

Corrigendum to "Is loneliness related to greater use of DeepSeek as an AI companion? A study from China" [Asian J. Psychiatr. 117 (2026) 104845].

Asian journal of psychiatry·2026

Same author

Biobased Flame-Retardant Epoxy Adhesive Based on Resveratrol: Phosphorus-Nitrogen Synergistic Flame Retardancy and Property Investigation.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Synthetic Control over the Electron-Beam Stability of Upconverting Nanoparticles.

Nano letters·2026

Same journal

Rapid and Continuous Directed Evolution in <i>Vibrio natriegens</i> Utilizing an <i>In Vivo</i> Hypermutation System.

ACS synthetic biology·2026

Same journal

Machine Learning for Microbial Cell Factories: Pathway Design, Enzyme Engineering, and Metabolic Regulation.

ACS synthetic biology·2026

Same journal

Microfluidics-Based Engineering of Molecular Self-Assembly and Manufacturing for Artificial Cell Systems.

ACS synthetic biology·2026

Same journal

Beyond Compartmentalization: Deciphering Reaction Kinetics in Liquid-Liquid Phase Separation for Rational Biotechnological Design.

ACS synthetic biology·2026

Same journal

Continuous Hypermutation and Evolution of Noncanonical Amino Acid Synthases.

ACS synthetic biology·2026

Same journal

Genome-Scale Community Models for Designing Efficient Lignocellulolytic Bacterial Consortia Using Bovine Rumen Microbes.

ACS synthetic biology·2026

See all related articles

Search research articles

Home
Engineering Escherichia Coli For Urease-driven Synthesis Of Metal Oxide Nanomaterials.

Home
Engineering Escherichia Coli For Urease-driven Synthesis Of Metal Oxide Nanomaterials.

Related Experiment Video

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

Engineering Escherichia coli for Urease-Driven Synthesis of Metal Oxide Nanomaterials.

Zong-Yen Wu^1,2, Alex Y W Lin³, Isaak E Müller¹

¹The U.S. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

ACS Synthetic Biology

|June 8, 2026

View abstract on PubMed

Summary

This summary is machine-generated.

Engineered bacteria can now produce various nanomaterials, including metal oxides, offering a sustainable and eco-friendly alternative to traditional methods. This breakthrough utilizes urease-based biomineralization for green synthesis.

Keywords:

CRAGE-Duet genome integration engineered Escherichia coli metal oxide nanoparticles programmable nanomaterial synthesis sustainable biomanufacturing urease-mediated biomineralization

More Related Videos

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Published on: November 27, 2015

Related Experiment Videos

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Reverse Microemulsion-mediated Synthesis of Monometallic and Bimetallic Early Transition Metal Carbide and Nitride Nanoparticles

Published on: November 27, 2015

Area of Science:

Biotechnology
Materials Science
Nanotechnology

Background:

Conventional nanomaterial synthesis is energy-intensive and environmentally challenging.
Urease-based biomineralization offers a sustainable, mild-condition alternative.
Controlled nanomaterial morphology is crucial for diverse applications.

Purpose of the Study:

To engineer Escherichia coli (E. coli) for urease-based green nanomaterial synthesis.
To demonstrate the versatility of E. coli as a platform for producing various inorganic nanomaterials.
To explore eco-friendly alternatives for nanomaterial production.

Main Methods:

Engineered E. coli to express the urease gene cluster from Sporosarcina pasteurii using CRAGE-Duet technology.
Utilized urease activity for biomineralization of calcium carbonate and calcium phosphate.

Synthesized metal oxide nanoparticles, including hematite (Fe2O3) and titanium dioxide (TiO2).

Characterized synthesized nanomaterials using electron microscopy.

Main Results:

Successfully produced calcium carbonate and calcium phosphate crystals via engineered E. coli.
Synthesized metal oxide nanoparticles: hematite (Fe2O3) and titanium dioxide (TiO2).
Electron microscopy confirmed the morphology and nature of the synthesized nanomaterials.

Conclusions:

Engineered E. coli serves as a sustainable and versatile platform for green nanomaterial synthesis.
Urease-based biomineralization in E. coli enables efficient production of diverse inorganic nanomaterials.
This approach presents a promising eco-friendly alternative for future nanomaterial manufacturing.