Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generative Artificial Intelligence and Large Language Models in Clinical Oncology.

MedComm·2026
Same author

Deep knowledge-driven multi-modal fusion for diagnosis and prognosis of SI-ARDS.

Communications medicine·2026
Same author

Fulvestrant versus capecitabine as maintenance therapy in hormone receptor-positive, HER2-negative metastatic breast cancer after first-line chemotherapy (FAMILY): a multicenter, open-label, randomized, phase 3 trial.

Signal transduction and targeted therapy·2026
Same author

Single-cell RNA sequencing identifies CD8Teff cell activation as a predictive biomarker in triple-negative breast cancer immunotherapy.

Molecular biomedicine·2025
Same author

Artificial Intelligence-Based Multimodal Prediction of Postoperative Adjuvant Immunotherapy Benefit in Urothelial Carcinoma: Results From the Phase III, Multicenter, Randomized, IMvigor010 Trial.

MedComm·2025
Same author

Contrast-enhanced ultrasound enables precision diagnosis of preoperative muscle invasion in bladder cancer: a prospective study.

MedComm·2025

Related Experiment Video

Updated: Jun 9, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Single-Cell and Spatial Transcriptomics Uncover Immune Dynamics and Cellular Heterogeneity in Benign Prostatic

Yuanyuan Luo1,2, Haitao Zhong3, Tongrui Shang3

  • 1Department of Urology Guizhou Provincial People's Hospital Guiyang Guizhou China.

Medcomm
|June 8, 2026
PubMed
Summary

Benign prostatic hyperplasia (BPH) progression to prostate cancer (PCa) involves cellular changes and a suppressive immune microenvironment. Understanding these dynamics aids early diagnosis and personalized immunotherapies for PCa.

Keywords:
benign prostatic hyperplasiacontinuous transformation mapimmune microenvironmentprostate cancersingle‐cell RNA sequencingspatial transcriptomics

More Related Videos

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
10:38

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Related Experiment Videos

Last Updated: Jun 9, 2026

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
10:38

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Area of Science:

  • Oncology
  • Immunology
  • Cell Biology

Background:

  • Prostate cancer (PCa) often co-occurs with benign prostatic hyperplasia (BPH).
  • The relationship between BPH, PCa progression, and immune status requires further investigation for improved early diagnosis and treatment.
  • Understanding the cellular and immune landscape is crucial for PCa management.

Purpose of the Study:

  • To analyze the association between normal, BPH, and PCa tissues using advanced transcriptomic techniques.
  • To map the cellular transformation from hyperplasia to malignancy.
  • To characterize the immune microenvironment during PCa development.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) of normal, BPH, and PCa tissues.
  • Spatial transcriptomics to analyze tissue architecture and cellular interactions.
  • In-depth analysis of immune cell populations (T cells, macrophages, NK cells, B cells) and their functions.

Main Results:

  • A continuous transformation map of luminal epithelial cells from hyperplasia to malignancy was identified.
  • A persistently suppressive immune microenvironment was observed in BPH and PCa tissues.
  • Infiltrated T cells exhibited exhaustion, macrophages showed M2 polarization and immune escape activation, and NK/B cells had limited function due to low abundance.

Conclusions:

  • The study reveals dynamic immune landscape changes during PCa occurrence and progression.
  • Classification and prognostic models offer a basis for immunomodulatory and personalized PCa therapies.
  • New tools for early diagnosis and intervention of PCa are provided.