Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Restriction Enzymes

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evolving a fluorescent light-up aptamer for rotationally quenched asymmetric rhodamines.

Chemical communications (Cambridge, England)·2026

Same author

A Near-Infrared Aptamer:Dye System For Live-Cell Super-Resolution RNA Imaging.

Journal of the American Chemical Society·2026

Same author

Single-step discovery of high-affinity RNA ligands by UltraSelex.

Nature chemical biology·2025

Same author

Synthesis and Development of Inverse-Type Nucleoside Diarylethene Photoswitches.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024

Same author

Identification and in vitro characterization of UDP-GlcNAc-RNA cap-modifying and decapping enzymes.

Nucleic acids research·2024

Same author

Identification of NAD-RNA species and ADPR-RNA decapping in Archaea.

Nature communications·2023

Same journal

Pressure-Related Challenges and Strategic Approaches in Lithium Metal Sulfide all-Solid-State Batteries.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Dual Regulatory Functions of Classical Zinc Finger Clusters from Myeloid Zinc Finger-1.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

A Selectfluor-based Polonovski Rearrangement Leading to Novel Entities for Synthetic and Medicinal Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Relay Approach: A Convergent Synthesis of Key Fragments en route to (+)-Neosorangicin A.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Working Under Pressure: Empirical Findings on the Challenges Facing PhD Students in Chemistry.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Charge Resonance Interaction in Aromatic Trimer Radical Cations Revealed by IR Spectroscopy: The Case of Pyrrole Homo- and Heterotrimers.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Published on: July 25, 2025

Fully Reversible Regulation of 8-17 DNAzyme Activity Using Nucleoside-Based Diarylethenes.

Jörn Bargstedt¹, Simon M Büllmann¹, Sophia Conwell¹

¹Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|June 8, 2026

Summary

This summary is machine-generated.

Researchers developed light-controlled DNA enzymes using nucleoside-based diarylethenes. This allows precise, reversible control over RNA cleavage, offering a new tool for chemical biology applications.

Keywords:

8–17 DNAzyme diarylethenes nucleosides photochromic DNA reversible regulation

More Related Videos

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

Related Experiment Videos

Last Updated: Jun 9, 2026

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Published on: July 25, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Published on: April 16, 2021

Area of Science:

Chemical Biology
Molecular Biology
Biochemistry

Background:

Enzyme activity control is crucial in chemical biology.
Light offers noninvasive, precise external control over molecular function.
Diarylethenes (DAEs) are photoswitchable molecules.

Purpose of the Study:

To achieve light-dependent control of the RNA-cleaving deoxyribozyme (DNAzyme) 8-17 using DAEs.
To investigate the impact of DAE structure on DNAzyme regulation.
To establish a thermally robust and reversible photoswitchable DNAzyme system.

Main Methods:

Incorporation of DAE-modified 2'-deoxyuridines into the DNAzyme 8-17 at specific positions.
Photochemical characterization of DNAzyme activity under UV and visible light.
Assessment of thermal stability and cycling capability of the photoswitchable DNAzyme.

Main Results:

A specific DAE modification (dU-PhtBu at position 2.1) enabled efficient and reversible photocontrol.
Catalytic rate decreased ~11-fold in the UV-induced photostationary state (PSSUV) compared to the open form (OF).
Isolated open form (OF) and closed form (CF) showed ~200-fold activity difference; the switch was thermally robust and reusable.

Conclusions:

Nucleoside-based DAEs can effectively confer light-dependent regulation to DNAzymes.
Precise DAE structure is critical for achieving functional photocontrol, with minor changes abolishing regulation.
This work provides a robust, light-switchable DNAzyme with potential applications in chemical biology.