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Updated: Jun 10, 2026

Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice

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Combining Collagenase Injections with Exercise-Induced Mechanical Overload: A Mouse Model Simulating Overload Stress

Zeng-Qiao Zhang¹, Jie Li¹, Tian-Jun Zhai²

¹Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine.

Journal of Visualized Experiments : Jove

|June 8, 2026

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This study introduces a new mouse model for knee osteoarthritis (KOA) by combining cartilage degradation with mechanical stress. This model accurately mimics human KOA progression, aiding in the development of new treatments.

Area of Science:

Orthopedics and Biomedical Engineering
Translational Medicine
Musculoskeletal Research

Background:

Knee osteoarthritis (KOA) is a degenerative joint disease with complex causes, poorly replicated by current surgical animal models.
Existing models often induce acute trauma, failing to capture the gradual nature of human KOA pathogenesis.

Purpose of the Study:

To develop a novel, reproducible mouse model of KOA that simulates overload stress-induced degeneration.
To bridge the translational gap between animal models and human KOA by mimicking gradual pathogenesis.

Main Methods:

Synergistic intervention involving mild enzymatic cartilage degradation (type II collagenase) and sustained mechanical loading over four weeks.
Utilized a rotator-type fatigue apparatus for calibrated mechanical overexertion.

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Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles

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Last Updated: Jun 10, 2026

Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice

Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice

Published on: September 29, 2023

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles

Published on: June 2, 2023

Comprehensive validation through automated gait analysis, histomorphological evaluation (safranin O-fast green staining), and immunohistochemistry.

Main Results:

The dual-factor intervention induced severe locomotor deficits, including reduced stride length and width.
Histological analysis revealed progressive cartilage damage, surface defects, and elevated Osteoarthritis Research Society International (OARSI) scores, mirroring early-to-mid stage human KOA.
Demonstrated TRPV4 channel upregulation and COL2A1 reduction in articular chondrocytes.

Conclusions:

This minimally invasive model effectively replicates key pathological features of human KOA without acute surgical trauma.
Establishes a clinically relevant platform for investigating molecular mechanisms, pain phenotypes, and therapeutic interventions for early-stage osteoarthritis.