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Related Experiment Video

Updated: Jun 10, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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Published on: March 13, 2018

Multiplexed and Ultrasensitive Pathogens Detection with an Orthogonal Argonaute Circuit Based on Encoded Microsphere

Letian Li1,2, Mengjiao Wang1, Qinyu Wang3

  • 1State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning 116034, China.

Analytical Chemistry
|June 8, 2026
PubMed
Summary

We developed a novel biosensing platform for rapid, ultrasensitive detection of multiple pathogens. This technology offers a 10-fold improvement in sensitivity over PCR for enhanced public health monitoring.

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Last Updated: Jun 10, 2026

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Biosensing

Background:

  • Multiplexed and sensitive pathogen detection is vital for public health surveillance.
  • Existing methods often lack the sensitivity, speed, or multiplexing capability required for comprehensive pathogen screening.

Purpose of the Study:

  • To present a novel Clostridium butyricum Argonaute (CbAgo)-based guide DNA (gDNA)-target DNA-circuit (GTC) platform for multiplexed and ultrasensitive pathogen detection.
  • To enable rapid, user-friendly, and accurate identification of multiple pathogens without DNA amplification.

Main Methods:

  • Development of a CbAgo-based GTC platform utilizing encoded microsphere microscopic imaging.
  • Design of a user-friendly interface for customized gDNA screening and cleavage circuit design.
  • Application of spatial confinement and computer vision for simultaneous detection and signal decoding.

Main Results:

  • Achieved reduction in cross-hybridization below 0.032% and enhanced CbAgo cleavage efficiency by up to 157%.
  • Demonstrated ultrasensitive detection of three pathogenic bacteria (10^1 to 10^7 CFU/mL) in 60 real-world samples within 70 minutes.
  • Exhibited a 10-fold improvement in sensitivity (limit of detection down to 1 CFU/mL) and higher accuracy compared to quantitative polymerase chain reaction (qPCR).

Conclusions:

  • The GTC platform provides an intelligent biosensing strategy for multiplexed pathogen detection.
  • This technology represents a promising next-generation digital platform for public health monitoring and disease surveillance.