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Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Related Experiment Video

Updated: Jun 10, 2026

Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

Efficient lentivirus concentration using a tabletop centrifuge.

Eva Müller¹, Julia Boesner¹, Zehra Karaköse¹

¹Institute for the Research on HIV and AIDS-associated Diseases, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.

Molecular Biology Reports

|June 9, 2026

Summary

This summary is machine-generated.

A rapid 15-minute tabletop centrifuge method efficiently concentrates viruses, improving titers and infection rates. This cost-effective technique offers a valuable alternative to traditional, time-consuming methods for virus concentration.

Keywords:

Centrifugation Infection Lentivirus Virus concentration

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Area of Science:

Virology
Molecular Biology
Biotechnology

Background:

Efficient virus concentration is essential for gene delivery, drug screening, and infection studies.
Conventional methods like ultracentrifugation are costly and time-consuming.
Alternative methods often have limited availability or accessibility.

Purpose of the Study:

To develop a rapid and cost-effective virus concentration protocol.
To optimize virus concentration using a tabletop centrifuge.
To compare the efficiency of tabletop centrifugation with PEG precipitation.

Main Methods:

Virus concentration using a tabletop centrifuge at 16,100 g for 15 minutes.
Optimization of centrifugation time.
Comparison with Polyethylene Glycol (PEG) precipitation methods.

Main Results:

Tabletop centrifugation yielded higher virus titers than PEG precipitation.
Achieved superior infection efficiencies with the optimized centrifugation protocol.
Reduced centrifugation time to 15 minutes while maintaining effective virus enrichment.

Conclusions:

A 15-minute tabletop centrifugation is sufficient for robust virus concentration.
This method enhances viral titers and enables efficient medium exchange.
Provides a cost-effective and accessible alternative, especially for high-biosafety-level labs.